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- W2028205223 abstract "We have developed a spectrophotometric procedure, based on a combination of established methods, for the quantitative determination of alkyl and alk-1-enyl (plasmalogens) ether-linked glycerolipids. It depends upon the release of alkylglycerols and alk-1-enylglycerols from phospholipids by phospholipase C (Bacillus cereus) followed by saponification or by Vitride reduction of the phospholipids; aldehydes are subsequently formed and measured colorimetrically after reacting them with a fuchsin reagent. The total alkyl and alk-1-enyl content of glycerolipids is determined by oxidation of the sample with periodate to form aldehydes and alkylglycolic aldehydes. The O-alk-1-enyl lipid content is determined on a separate sample by measuring the aldehydes produced after acid hydrolysis. The quantity of O-alkyl lipids is calculated from the difference between the values obtained for the total ether-lipid content and that of the O-alk-1-enyl lipid content. Alternately, direct determination of alk-1-enylglycerols and alkylglycerols can be made if these hydrolytic products are first separated by thin-layer chromatography." @default.
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- W2028205223 date "1975-02-01" @default.
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- W2028205223 title "A method for the quantitative determination of glycerolipids containing O-alkyl and O-alk-1-enyl moieties" @default.
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- W2028205223 doi "https://doi.org/10.1016/0005-2760(75)90007-7" @default.
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