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• W2028235789 abstract "In this study the negatively charged Proteus vulgaris O25 LPS was chosen for studying interaction with polycationic chitosan. The complex formation of LPS with chitosan was demonstrated using gradient centrifugation and laser interferometry method. The presented results have shown that laser interferometry method is sensitive enough for LPS–chitosan interaction studies. The changing in the ultra structure of LPS during binding with chitosan was observed by electronic microscope. The interaction of P. vulgaris O25 LPS with chitosan was shown to modulate significantly the biological activities of LPS. The toxicity of P. vulgaris O25 LPS decreased 10-fold after forming complexes with chitosan at injection to mice in the similar concentration of endotoxin. The complex LPS–chitosan was less effective than LPS alone in Limulus amabocyte lysate assay. Induction of TNF biosynthesis by LPS–chitosan complex was found to be 65% lower than that by parent LPS at concentration of 100 ng/ml." @default.
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• W2028235789 date "2009-10-01" @default.
• W2028235789 modified "2023-10-16" @default.
• W2028235789 title "Binding and biological properties of lipopolysaccharide Proteus vulgaris O25 (48/57)–chitosan complexes" @default.
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• W2028235789 doi "https://doi.org/10.1016/j.carbpol.2009.05.004" @default.
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