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• W2028255158 abstract "Purification of the obligate intracellular bacterium Coxiella burnetii requires physical disruption of infected cells. Here we describe a gentle and safe digitonin lysis procedure to release C. burnetii from infected cells. The purity, yield, and infectivity of digitonin-prepped organisms are comparable to that of organisms purified using cell lysis by sonication." @default.
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• W2028255158 date "2008-03-01" @default.
• W2028255158 modified "2023-09-23" @default.
• W2028255158 title "A method for purifying obligate intracellular Coxiella burnetii that employs digitonin lysis of host cells" @default.
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• W2028255158 doi "https://doi.org/10.1016/j.mimet.2007.12.015" @default.
• W2028255158 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2276870" @default.
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