Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028257782> ?p ?o ?g. }
- W2028257782 endingPage "43" @default.
- W2028257782 startingPage "37" @default.
- W2028257782 abstract "Japanese encephalitis (JE) is a disease that threatens both human and animal populations in Asian countries, and the causative agent of JE, Japanese encephalitis virus (JEV), has recently changed from genotype III (GIII) to genotype I (GI). However, a test for the rapid differentiation of GI and GIII JEV is still unavailable, especially one that can be used for mosquito-based surveillance. We have designed GI- and GIII-specific primer sets for the rapid detection and differentiation of GI and GIII JEV by multiplex reverse transcriptase-polymerase chain reaction (multiplex RT-PCR). The GI-specific and GIII-specific primer sets were able to specifically amplify the target gene from GI and GIII JEV, respectively. The limitations of detection were 0.00225 and 0.225 pfu for the GI-specific and GIII-specific primers, respectively. Using a mixture of GI-specific and GIII-specific primers, the multiplex RT-PCR was able to specifically detect and differentiate GI and GIII JEV. The multiplex RT-PCR was able to successfully differentiate GI and GIII virus in JEV-infected mosquitoes. Thus, a sensitive and specific multiplex RT-PCR system for the rapid detection and differentiation of GI and GIII JEV has been developed, and this test is likely to be valuable when carrying out mosquito-based JEV surveillance." @default.
- W2028257782 created "2016-06-24" @default.
- W2028257782 creator A5024883783 @default.
- W2028257782 creator A5070640773 @default.
- W2028257782 creator A5088683792 @default.
- W2028257782 creator A5089921899 @default.
- W2028257782 date "2012-11-16" @default.
- W2028257782 modified "2023-09-27" @default.
- W2028257782 title "Detection and Differentiation of Genotype I and III Japanese Encephalitis Virus in Mosquitoes by Multiplex Reverse Transcriptase-Polymerase Chain Reaction" @default.
- W2028257782 cites W1993651229 @default.
- W2028257782 cites W2000179600 @default.
- W2028257782 cites W2007499375 @default.
- W2028257782 cites W2012872626 @default.
- W2028257782 cites W2034632458 @default.
- W2028257782 cites W2037463365 @default.
- W2028257782 cites W2038856465 @default.
- W2028257782 cites W2062190635 @default.
- W2028257782 cites W2072252766 @default.
- W2028257782 cites W2079953254 @default.
- W2028257782 cites W2086669908 @default.
- W2028257782 cites W2106584592 @default.
- W2028257782 cites W2110441168 @default.
- W2028257782 cites W2110721832 @default.
- W2028257782 cites W2117676129 @default.
- W2028257782 cites W2118441980 @default.
- W2028257782 cites W2120866886 @default.
- W2028257782 cites W2123009908 @default.
- W2028257782 cites W2129421877 @default.
- W2028257782 cites W2137697889 @default.
- W2028257782 cites W2142855881 @default.
- W2028257782 cites W2145137798 @default.
- W2028257782 cites W2147060276 @default.
- W2028257782 cites W2153309140 @default.
- W2028257782 cites W2165902807 @default.
- W2028257782 cites W225171105 @default.
- W2028257782 doi "https://doi.org/10.1111/j.1865-1682.2012.01369.x" @default.
- W2028257782 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23157736" @default.
- W2028257782 hasPublicationYear "2012" @default.
- W2028257782 type Work @default.
- W2028257782 sameAs 2028257782 @default.
- W2028257782 citedByCount "10" @default.
- W2028257782 countsByYear W20282577822014 @default.
- W2028257782 countsByYear W20282577822015 @default.
- W2028257782 countsByYear W20282577822016 @default.
- W2028257782 countsByYear W20282577822017 @default.
- W2028257782 countsByYear W20282577822018 @default.
- W2028257782 countsByYear W20282577822020 @default.
- W2028257782 countsByYear W20282577822021 @default.
- W2028257782 countsByYear W20282577822022 @default.
- W2028257782 crossrefType "journal-article" @default.
- W2028257782 hasAuthorship W2028257782A5024883783 @default.
- W2028257782 hasAuthorship W2028257782A5070640773 @default.
- W2028257782 hasAuthorship W2028257782A5088683792 @default.
- W2028257782 hasAuthorship W2028257782A5089921899 @default.
- W2028257782 hasBestOaLocation W20282577821 @default.
- W2028257782 hasConcept C104317684 @default.
- W2028257782 hasConcept C135763542 @default.
- W2028257782 hasConcept C153911025 @default.
- W2028257782 hasConcept C156719811 @default.
- W2028257782 hasConcept C159047783 @default.
- W2028257782 hasConcept C178790620 @default.
- W2028257782 hasConcept C185592680 @default.
- W2028257782 hasConcept C2522874641 @default.
- W2028257782 hasConcept C2777563447 @default.
- W2028257782 hasConcept C2779141303 @default.
- W2028257782 hasConcept C2781188995 @default.
- W2028257782 hasConcept C49105822 @default.
- W2028257782 hasConcept C54355233 @default.
- W2028257782 hasConcept C83455156 @default.
- W2028257782 hasConcept C86803240 @default.
- W2028257782 hasConcept C90583042 @default.
- W2028257782 hasConceptScore W2028257782C104317684 @default.
- W2028257782 hasConceptScore W2028257782C135763542 @default.
- W2028257782 hasConceptScore W2028257782C153911025 @default.
- W2028257782 hasConceptScore W2028257782C156719811 @default.
- W2028257782 hasConceptScore W2028257782C159047783 @default.
- W2028257782 hasConceptScore W2028257782C178790620 @default.
- W2028257782 hasConceptScore W2028257782C185592680 @default.
- W2028257782 hasConceptScore W2028257782C2522874641 @default.
- W2028257782 hasConceptScore W2028257782C2777563447 @default.
- W2028257782 hasConceptScore W2028257782C2779141303 @default.
- W2028257782 hasConceptScore W2028257782C2781188995 @default.
- W2028257782 hasConceptScore W2028257782C49105822 @default.
- W2028257782 hasConceptScore W2028257782C54355233 @default.
- W2028257782 hasConceptScore W2028257782C83455156 @default.
- W2028257782 hasConceptScore W2028257782C86803240 @default.
- W2028257782 hasConceptScore W2028257782C90583042 @default.
- W2028257782 hasIssue "1" @default.
- W2028257782 hasLocation W20282577821 @default.
- W2028257782 hasLocation W20282577822 @default.
- W2028257782 hasOpenAccess W2028257782 @default.
- W2028257782 hasPrimaryLocation W20282577821 @default.
- W2028257782 hasRelatedWork W1981191399 @default.
- W2028257782 hasRelatedWork W2028257782 @default.
- W2028257782 hasRelatedWork W2049614586 @default.
- W2028257782 hasRelatedWork W2067557457 @default.
- W2028257782 hasRelatedWork W2080534576 @default.
- W2028257782 hasRelatedWork W2302132233 @default.