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- W2028282700 abstract "Intracellular modifications of anionic photosensitizers during PDT were observed, dependent on the growth phase of the incubated cells. In addition to steady-state spectroscopy, time- resolved fluorescence spectra between different time gates were measured. Comparative results were obtained for the sensitizers meso-tetra(4-sulfonatophenyl)porphyrine (TPPS4) and aluminumsulfophthalocyanine (AlS2-3Pc). In both cases relocalization of the sensitizer could be observed exclusively for cells in the growing phase, whereas no relocalization was detected for cells in the stationary phase. Moreover, time-gated spectra were different for the two cell types. The formation of a fluorescent band around 615 nm during PDT for the sensitizer TPPS4 was observed mainly for cells in the growing phase. This species was correlated with a short fluorescence decay time (< 5 ns). Similar observations were done with AlS2-3Pc. A fluorescence band blue-shifted in the same order of magnitude (about 30 nm from the original band) and measured predominantly between an `early' time-gate (0-5 ns) was detected for cells in the growing phase.© (1995) COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only." @default.
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- W2028282700 date "1995-02-01" @default.
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- W2028282700 title "<title>Intracellular relocalization of anionic photosensitizers measured by cw and time-gated microscopy and spectroscopy</title>" @default.
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- W2028282700 doi "https://doi.org/10.1117/12.200888" @default.
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