FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028305369> ?p ?o ?g. }

• W2028305369 endingPage "396" @default.
• W2028305369 startingPage "389" @default.
• W2028305369 abstract "Abstract. Arginine-specific mono-ADP-ribosylation of proteins and arginine-specific mono-ADP-ribosyltransferase occur in heart. We developed a polyclonal antiserum, R-28, against ADP-ribosylpolyarginine that recognized mono-ADP-ribosylated proteins and identified the major mono-ADP-ribosylation products of quail heart. Treatment of Immobilon-bound ADP-ribosylated Gs protein with hydroxylamine under conditions that remove ADP-ribose from its arginines eliminated R-28 immunoreactivity to Gs. Also, R-28 immunoreactivity to quail heart proteins was removed by NaOH and phosphodiesterase I treatments. Similar treatment with mercuric chloride did not remove the immunoreactivity but did remove exogenously (via in vitro pertussis toxin treatment) added ADP-ribose from cysteine of cardiac Gi/Go proteins. The antiserum did not appear to react with ADP-ribosylasparagine of Rho (formed by C3 toxin), ADP-ribosyldiphthamide of elongation factor 2 (formed by diphtheria toxin) in quail heart preparations, or polyADP-ribosylated proteins of a neonate rat cardiac nuclear preparation. Thus, the R-28 antiserum appears to contain predominantly antibodies directed against ADP-ribosylarginine. To test the usefulness of R-28, immunoblotting of subcellular fractions of quail heart was performed. R-28 showed the greatest immunoreactivity in the sarcolemma with significant immunoreactivity in denser membrane fractions. The cytosol also contained an immunoreactive band distinct from those found in the membranes. Hydroxylamine treatment eliminated immunoreactivity in the sarcolemma and denser membrane fractions but not the cytosol, suggesting the membranous immunoreactive bands contain ADP-ribosylarginine. In conclusion, a polyclonal antiserum that recognizes ADP-ribosylarginine proteins has been raised. The usefulness of the antiserum is demonstrated by the characterization of endogenous arginine mono-ADP-ribosylation products in quail heart. The quail heart has several sarcolemmal and denser membrane fraction proteins that appear to be mono-ADP-ribosylated on arginines." @default.
• W2028305369 created "2016-06-24" @default.
• W2028305369 creator A5011200834 @default.
• W2028305369 creator A5015602544 @default.
• W2028305369 creator A5063232705 @default.
• W2028305369 creator A5065985641 @default.
• W2028305369 date "2008-07-18" @default.
• W2028305369 modified "2023-10-06" @default.
• W2028305369 title "Evidence of Endogenous Mono-ADP-Ribosylation of Cardiac Proteins Via Anti-ADP-Ribosylarginine Immunoreactivity" @default.
• W2028305369 cites W1012766462 @default.
• W2028305369 cites W1015369645 @default.
• W2028305369 cites W1525126648 @default.
• W2028305369 cites W1568450501 @default.
• W2028305369 cites W186308413 @default.
• W2028305369 cites W1968364733 @default.
• W2028305369 cites W1968467509 @default.
• W2028305369 cites W2017238824 @default.
• W2028305369 cites W2027730661 @default.
• W2028305369 cites W2028188139 @default.
• W2028305369 cites W2040080953 @default.
• W2028305369 cites W2040561530 @default.
• W2028305369 cites W2068945068 @default.
• W2028305369 cites W2090774820 @default.
• W2028305369 cites W2213194893 @default.
• W2028305369 cites W2236981936 @default.
• W2028305369 cites W2344464612 @default.
• W2028305369 cites W82323476 @default.
• W2028305369 doi "https://doi.org/10.1111/j.1525-1373.2000.22355.x" @default.
• W2028305369 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10721009" @default.
• W2028305369 hasPublicationYear "2008" @default.
• W2028305369 type Work @default.
• W2028305369 sameAs 2028305369 @default.
• W2028305369 citedByCount "3" @default.
• W2028305369 countsByYear W20283053692014 @default.
• W2028305369 countsByYear W20283053692018 @default.
• W2028305369 crossrefType "journal-article" @default.
• W2028305369 hasAuthorship W2028305369A5011200834 @default.
• W2028305369 hasAuthorship W2028305369A5015602544 @default.
• W2028305369 hasAuthorship W2028305369A5063232705 @default.
• W2028305369 hasAuthorship W2028305369A5065985641 @default.
• W2028305369 hasConcept C134018914 @default.
• W2028305369 hasConcept C153911025 @default.
• W2028305369 hasConcept C159654299 @default.
• W2028305369 hasConcept C170493617 @default.
• W2028305369 hasConcept C181199279 @default.
• W2028305369 hasConcept C199096232 @default.
• W2028305369 hasConcept C203014093 @default.
• W2028305369 hasConcept C2777367657 @default.
• W2028305369 hasConcept C2777468819 @default.
• W2028305369 hasConcept C2777882087 @default.
• W2028305369 hasConcept C33057221 @default.
• W2028305369 hasConcept C41625074 @default.
• W2028305369 hasConcept C45510557 @default.
• W2028305369 hasConcept C49773422 @default.
• W2028305369 hasConcept C515207424 @default.
• W2028305369 hasConcept C55493867 @default.
• W2028305369 hasConcept C75520062 @default.
• W2028305369 hasConcept C80631254 @default.
• W2028305369 hasConcept C86803240 @default.
• W2028305369 hasConcept C91523082 @default.
• W2028305369 hasConcept C956191 @default.
• W2028305369 hasConcept C98539663 @default.
• W2028305369 hasConceptScore W2028305369C134018914 @default.
• W2028305369 hasConceptScore W2028305369C153911025 @default.
• W2028305369 hasConceptScore W2028305369C159654299 @default.
• W2028305369 hasConceptScore W2028305369C170493617 @default.
• W2028305369 hasConceptScore W2028305369C181199279 @default.
• W2028305369 hasConceptScore W2028305369C199096232 @default.
• W2028305369 hasConceptScore W2028305369C203014093 @default.
• W2028305369 hasConceptScore W2028305369C2777367657 @default.
• W2028305369 hasConceptScore W2028305369C2777468819 @default.
• W2028305369 hasConceptScore W2028305369C2777882087 @default.
• W2028305369 hasConceptScore W2028305369C33057221 @default.
• W2028305369 hasConceptScore W2028305369C41625074 @default.
• W2028305369 hasConceptScore W2028305369C45510557 @default.
• W2028305369 hasConceptScore W2028305369C49773422 @default.
• W2028305369 hasConceptScore W2028305369C515207424 @default.
• W2028305369 hasConceptScore W2028305369C55493867 @default.
• W2028305369 hasConceptScore W2028305369C75520062 @default.
• W2028305369 hasConceptScore W2028305369C80631254 @default.
• W2028305369 hasConceptScore W2028305369C86803240 @default.
• W2028305369 hasConceptScore W2028305369C91523082 @default.
• W2028305369 hasConceptScore W2028305369C956191 @default.
• W2028305369 hasConceptScore W2028305369C98539663 @default.
• W2028305369 hasIssue "4" @default.
• W2028305369 hasLocation W20283053691 @default.
• W2028305369 hasOpenAccess W2028305369 @default.
• W2028305369 hasPrimaryLocation W20283053691 @default.
• W2028305369 hasRelatedWork W1977900662 @default.
• W2028305369 hasRelatedWork W2016094282 @default.
• W2028305369 hasRelatedWork W2028305369 @default.
• W2028305369 hasRelatedWork W2369348973 @default.
• W2028305369 hasRelatedWork W2389887198 @default.
• W2028305369 hasRelatedWork W2390680447 @default.
• W2028305369 hasRelatedWork W2398355123 @default.
• W2028305369 hasRelatedWork W2462039668 @default.
• W2028305369 hasRelatedWork W4243635366 @default.
• W2028305369 hasRelatedWork W4249158774 @default.

• next