Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028355032> ?p ?o ?g. }
- W2028355032 endingPage "1160" @default.
- W2028355032 startingPage "1153" @default.
- W2028355032 abstract "Pulmonary neuroepithelial bodies (NEBs) are densely innervated groups of complex sensory airway receptors involved in the regulation of breathing. Together with their surrounding Clara-like cells, they exhibit stem cell potential through their capacity to regenerate depopulated areas of the epithelium following lung injury. We have employed confocal live cell imaging microscopy and novel electrophysiological techniques in a new ex vivo lung slice model to unravel potential purinergic signaling pathways within the NEB microenvironment. Quinacrine histochemistry indicated high amounts of vesicular ATP in NEB cells. Using a reporter-patching method adapted to create a uniquely sensitive and selective biosensor for the direct detection of ATP release from NEBs ex vivo, we demonstrated quantal ATP release from NEBs following their depolarization. Enhancing enzymatic extracellular ATP hydrolysis or inhibiting P2 receptors confirmed the central role of ATP in paracrine interactions between NEB cells and Clara-like cells. Combined calcium imaging, pharmacology, and immunohistochemistry showed that ligand-binding to functional P2Y(2) receptors underpins the activation of Clara-like cells. Hence, NEB cells communicate with their cellular neighbors in the NEB microenvironment by releasing ATP, which rapidly evokes purinergic activation of surrounding Clara-like cells. Besides ATP acting on the P2X(3) receptor expressing vagal sensory nerve terminals between NEB cells, local paracrine purinergic signaling within this potential stem cell niche may be important to both normal airway function, airway epithelial regeneration after injury, and/or the pathogenesis of small cell lung carcinomas." @default.
- W2028355032 created "2016-06-24" @default.
- W2028355032 creator A5000207645 @default.
- W2028355032 creator A5008950728 @default.
- W2028355032 creator A5018352656 @default.
- W2028355032 creator A5024901856 @default.
- W2028355032 creator A5043500725 @default.
- W2028355032 creator A5046890123 @default.
- W2028355032 creator A5062022173 @default.
- W2028355032 creator A5067821485 @default.
- W2028355032 creator A5072905988 @default.
- W2028355032 creator A5085832818 @default.
- W2028355032 date "2008-12-02" @default.
- W2028355032 modified "2023-10-16" @default.
- W2028355032 title "Purinergic signaling in the pulmonary neuroepithelial body microenvironment unraveled by live cell imaging" @default.
- W2028355032 cites W1485797978 @default.
- W2028355032 cites W1900866816 @default.
- W2028355032 cites W1963715285 @default.
- W2028355032 cites W1964403908 @default.
- W2028355032 cites W1968490089 @default.
- W2028355032 cites W1978241980 @default.
- W2028355032 cites W1981454105 @default.
- W2028355032 cites W1982358958 @default.
- W2028355032 cites W1995292337 @default.
- W2028355032 cites W1995810932 @default.
- W2028355032 cites W2003487397 @default.
- W2028355032 cites W2012336208 @default.
- W2028355032 cites W2015362146 @default.
- W2028355032 cites W2019408299 @default.
- W2028355032 cites W2020255875 @default.
- W2028355032 cites W2024596688 @default.
- W2028355032 cites W2027077773 @default.
- W2028355032 cites W2029100084 @default.
- W2028355032 cites W2031786722 @default.
- W2028355032 cites W2034770332 @default.
- W2028355032 cites W2042185114 @default.
- W2028355032 cites W2046079214 @default.
- W2028355032 cites W2050330888 @default.
- W2028355032 cites W2065009203 @default.
- W2028355032 cites W2065773356 @default.
- W2028355032 cites W2074869825 @default.
- W2028355032 cites W2080311098 @default.
- W2028355032 cites W2108236779 @default.
- W2028355032 cites W2114738541 @default.
- W2028355032 cites W2119835285 @default.
- W2028355032 cites W2129226540 @default.
- W2028355032 cites W2130244999 @default.
- W2028355032 cites W2130752844 @default.
- W2028355032 cites W2134498162 @default.
- W2028355032 cites W2139379166 @default.
- W2028355032 cites W2145505569 @default.
- W2028355032 cites W2183321532 @default.
- W2028355032 cites W2417095020 @default.
- W2028355032 cites W54538561 @default.
- W2028355032 cites W963764839 @default.
- W2028355032 doi "https://doi.org/10.1096/fj.08-109579" @default.
- W2028355032 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19050048" @default.
- W2028355032 hasPublicationYear "2008" @default.
- W2028355032 type Work @default.
- W2028355032 sameAs 2028355032 @default.
- W2028355032 citedByCount "42" @default.
- W2028355032 countsByYear W20283550322012 @default.
- W2028355032 countsByYear W20283550322013 @default.
- W2028355032 countsByYear W20283550322014 @default.
- W2028355032 countsByYear W20283550322015 @default.
- W2028355032 countsByYear W20283550322017 @default.
- W2028355032 countsByYear W20283550322018 @default.
- W2028355032 countsByYear W20283550322020 @default.
- W2028355032 countsByYear W20283550322021 @default.
- W2028355032 countsByYear W20283550322022 @default.
- W2028355032 countsByYear W20283550322023 @default.
- W2028355032 crossrefType "journal-article" @default.
- W2028355032 hasAuthorship W2028355032A5000207645 @default.
- W2028355032 hasAuthorship W2028355032A5008950728 @default.
- W2028355032 hasAuthorship W2028355032A5018352656 @default.
- W2028355032 hasAuthorship W2028355032A5024901856 @default.
- W2028355032 hasAuthorship W2028355032A5043500725 @default.
- W2028355032 hasAuthorship W2028355032A5046890123 @default.
- W2028355032 hasAuthorship W2028355032A5062022173 @default.
- W2028355032 hasAuthorship W2028355032A5067821485 @default.
- W2028355032 hasAuthorship W2028355032A5072905988 @default.
- W2028355032 hasAuthorship W2028355032A5085832818 @default.
- W2028355032 hasConcept C170493617 @default.
- W2028355032 hasConcept C172590197 @default.
- W2028355032 hasConcept C185592680 @default.
- W2028355032 hasConcept C2778938600 @default.
- W2028355032 hasConcept C2780410667 @default.
- W2028355032 hasConcept C28406088 @default.
- W2028355032 hasConcept C529295009 @default.
- W2028355032 hasConcept C54355233 @default.
- W2028355032 hasConcept C55493867 @default.
- W2028355032 hasConcept C57306754 @default.
- W2028355032 hasConcept C67907053 @default.
- W2028355032 hasConcept C7876069 @default.
- W2028355032 hasConcept C86803240 @default.
- W2028355032 hasConcept C87568996 @default.
- W2028355032 hasConcept C95444343 @default.
- W2028355032 hasConceptScore W2028355032C170493617 @default.