FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028357718> ?p ?o ?g. }

• W2028357718 endingPage "119" @default.
• W2028357718 startingPage "111" @default.
• W2028357718 abstract "So far, 7 different genes of the β-substituted alanine synthase family coding for proteins with O-acetyl-L-serine(thiol)lyase activity (OAS-TL) have been identified in Arabidopsis. The differences with respect to function and regulation of these isoforms are not well understood. In the present work, we aimed to differentiate between three OAS-TL proteins, A, B, and C, which are presumably localized in the cytoplasm, in plastids, and in mitochondria, respectively. The mature proteins were expressed in E. coli and the pH optima for the formation of cysteine were determined to pH 8.0, pH 8.0, and pH 7.0, respectively. The purified recombinant OAS-TL proteins also catalysed the formation of hydrogen sulfide (H2S) and β-cyanoalanine from cysteine in the presence of dithiothreitol or KCN, respectively. The three isoenzymes differed in their Km for cysteine in the presence of dithiothreitol, but the Km for cysteine in the presence of KCN were almost identical. On a molar basis, the formation of H2S or cysteine by the OAS-TL proteins varied in relation to pH. For the determination of both enzyme activities, cysteine synthesis and cysteine desulfuration, in different compartments of plant cells, chloroplasts and mitochondria were isolated from Arabidopsis plants and the OAS-TL and L-cysteine desulfhydrase activities were determined. In conclusion, a) the evolution of H2S from higher plants originates from different enzyme reactions, and b) the side reactions of the OAS-TL proteins seem to contribute massively to the total H2S release of higher plants at least at higher pH values." @default.
• W2028357718 created "2016-06-24" @default.
• W2028357718 creator A5029856459 @default.
• W2028357718 creator A5031985350 @default.
• W2028357718 creator A5061572630 @default.
• W2028357718 date "2002-01-01" @default.
• W2028357718 modified "2023-10-18" @default.
• W2028357718 title "Three O -acetyl-L-serine(thiol)lyase isoenzymes from Arabidopsis catalyse cysteine synthesis and cysteine desulfuration at different pH values" @default.
• W2028357718 cites W1571136093 @default.
• W2028357718 cites W1574115447 @default.
• W2028357718 cites W1580308416 @default.
• W2028357718 cites W1603278060 @default.
• W2028357718 cites W1655445913 @default.
• W2028357718 cites W1955897922 @default.
• W2028357718 cites W1963775955 @default.
• W2028357718 cites W1974302254 @default.
• W2028357718 cites W1984226402 @default.
• W2028357718 cites W1990503857 @default.
• W2028357718 cites W1993610637 @default.
• W2028357718 cites W1993921350 @default.
• W2028357718 cites W1996107394 @default.
• W2028357718 cites W2031249501 @default.
• W2028357718 cites W2033664430 @default.
• W2028357718 cites W2037763413 @default.
• W2028357718 cites W2039358903 @default.
• W2028357718 cites W2041265350 @default.
• W2028357718 cites W2041454096 @default.
• W2028357718 cites W2042642999 @default.
• W2028357718 cites W2049998464 @default.
• W2028357718 cites W2060121377 @default.
• W2028357718 cites W2060530787 @default.
• W2028357718 cites W2064216385 @default.
• W2028357718 cites W2067486816 @default.
• W2028357718 cites W2072261575 @default.
• W2028357718 cites W2075326198 @default.
• W2028357718 cites W2077400521 @default.
• W2028357718 cites W2078491857 @default.
• W2028357718 cites W2087921651 @default.
• W2028357718 cites W2100193053 @default.
• W2028357718 cites W2100837269 @default.
• W2028357718 cites W2117105947 @default.
• W2028357718 cites W2118414632 @default.
• W2028357718 cites W2131942711 @default.
• W2028357718 cites W2141576994 @default.
• W2028357718 cites W2158625473 @default.
• W2028357718 cites W2608717872 @default.
• W2028357718 cites W2886366794 @default.
• W2028357718 cites W4241771146 @default.
• W2028357718 cites W4247022189 @default.
• W2028357718 cites W4254166105 @default.
• W2028357718 cites W4293247451 @default.
• W2028357718 cites W8906737 @default.
• W2028357718 doi "https://doi.org/10.1078/0176-1617-00611" @default.
• W2028357718 hasPublicationYear "2002" @default.
• W2028357718 type Work @default.
• W2028357718 sameAs 2028357718 @default.
• W2028357718 citedByCount "22" @default.
• W2028357718 countsByYear W20283577182012 @default.
• W2028357718 countsByYear W20283577182015 @default.
• W2028357718 countsByYear W20283577182018 @default.
• W2028357718 countsByYear W20283577182021 @default.
• W2028357718 countsByYear W20283577182022 @default.
• W2028357718 countsByYear W20283577182023 @default.
• W2028357718 crossrefType "journal-article" @default.
• W2028357718 hasAuthorship W2028357718A5029856459 @default.
• W2028357718 hasAuthorship W2028357718A5031985350 @default.
• W2028357718 hasAuthorship W2028357718A5061572630 @default.
• W2028357718 hasConcept C104317684 @default.
• W2028357718 hasConcept C143065580 @default.
• W2028357718 hasConcept C16525657 @default.
• W2028357718 hasConcept C181199279 @default.
• W2028357718 hasConcept C185592680 @default.
• W2028357718 hasConcept C187566844 @default.
• W2028357718 hasConcept C2775988993 @default.
• W2028357718 hasConcept C2776414213 @default.
• W2028357718 hasConcept C2777269803 @default.
• W2028357718 hasConcept C2777824588 @default.
• W2028357718 hasConcept C2779201268 @default.
• W2028357718 hasConcept C2779491563 @default.
• W2028357718 hasConcept C2781139505 @default.
• W2028357718 hasConcept C2781324293 @default.
• W2028357718 hasConcept C55493867 @default.
• W2028357718 hasConcept C69305403 @default.
• W2028357718 hasConceptScore W2028357718C104317684 @default.
• W2028357718 hasConceptScore W2028357718C143065580 @default.
• W2028357718 hasConceptScore W2028357718C16525657 @default.
• W2028357718 hasConceptScore W2028357718C181199279 @default.
• W2028357718 hasConceptScore W2028357718C185592680 @default.
• W2028357718 hasConceptScore W2028357718C187566844 @default.
• W2028357718 hasConceptScore W2028357718C2775988993 @default.
• W2028357718 hasConceptScore W2028357718C2776414213 @default.
• W2028357718 hasConceptScore W2028357718C2777269803 @default.
• W2028357718 hasConceptScore W2028357718C2777824588 @default.
• W2028357718 hasConceptScore W2028357718C2779201268 @default.
• W2028357718 hasConceptScore W2028357718C2779491563 @default.
• W2028357718 hasConceptScore W2028357718C2781139505 @default.
• W2028357718 hasConceptScore W2028357718C2781324293 @default.
• W2028357718 hasConceptScore W2028357718C55493867 @default.

• next