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• W2028362476 abstract "The purpose of this study was to investigate the feasibility of cardiac microdialysis for the in vivo estimation of cardiac interstitial peptide concentrations, and, to determine the changes in neuropeptide Y release in myocardial tissue during experimental brain death in pigs. Using a specifically designed concentric flexible probe, perfused with Ringer solution containing 0.5% of bovine serum albumin at a flow rate of 2 μl/min, allowed us to obtain a 23 ± 2% relative recovery rate in vitro. Based on these in vitro recovery data, a regional study of the kinetics of interstitial NPY levels following brain death was obtained by monitoring the changes in NPY dialysate levels recorded from dialysis probes implanted into the right and left ventricular walls of the beating heart in vivo. Basal dialysate NPY levels determined by radioimmunoassay were of 95.2 ± 7.0 and 93.2 ± 9.1 pmol/l in left and right ventricle, respectively. Brain death was followed by a sustained 2 h increase in NPY dialysate levels in both ventricles (peak levels: 173.2 ± 30.9 pmol/l in left ventricle, and 149.7 ± 23.9 pmol/l in right ventricle), which then returned to control levels. We conclude that cardiac microdialysis is a simple and promising new tool for evaluating the role of peptides in cardiovascular regulation." @default.
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• W2028362476 date "1993-11-01" @default.
• W2028362476 modified "2023-10-10" @default.
• W2028362476 title "Microdialysis in the estimation of interstitial myocardial neuropeptide Y release" @default.
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• W2028362476 doi "https://doi.org/10.1016/0167-0115(93)90387-n" @default.
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