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• W2028363596 endingPage "e0117370" @default.
• W2028363596 startingPage "e0117370" @default.
• W2028363596 abstract "Xenopus is an excellent tetrapod model for studying normal and pathological motoneuron ontogeny due to its developmental morpho-physiological advantages. In mammals, the urotensin II-related peptide (UTS2B) gene is primarily expressed in motoneurons of the brainstem and the spinal cord. Here, we show that this expression pattern was conserved in Xenopus and established during the early embryonic development, starting at the early tailbud stage. In late tadpole stage, uts2b mRNA was detected both in the hindbrain and in the spinal cord. Spinal uts2b+ cells were identified as axial motoneurons. In adult, however, the uts2b expression was only detected in the hindbrain. We assessed the ability of the uts2b promoter to drive the expression of a fluorescent reporter in motoneurons by recombineering a green fluorescent protein (GFP) into a bacterial artificial chromosome (BAC) clone containing the entire X. tropicalis uts2b locus. After injection of this construction in one-cell stage embryos, a transient GFP expression was observed in the spinal cord of about a quarter of the resulting animals from the early tailbud stage and up to juveniles. The GFP expression pattern was globally consistent with that of the endogenous uts2b in the spinal cord but no fluorescence was observed in the brainstem. A combination of histological and electrophysiological approaches was employed to further characterize the GFP+ cells in the larvae. More than 98% of the GFP+ cells expressed choline acetyltransferase, while their projections were co-localized with α-bungarotoxin labeling. When tail myotomes were injected with rhodamine dextran amine crystals, numerous double-stained GFP+ cells were observed. In addition, intracellular electrophysiological recordings of GFP+ neurons revealed locomotion-related rhythmic discharge patterns during fictive swimming. Taken together our results provide evidence that uts2b is an appropriate driver to express reporter genes in larval motoneurons of the Xenopus spinal cord." @default.
• W2028363596 created "2016-06-24" @default.
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• W2028363596 date "2015-02-06" @default.
• W2028363596 modified "2023-10-18" @default.
• W2028363596 title "Generation of BAC Transgenic Tadpoles Enabling Live Imaging of Motoneurons by Using the Urotensin II-Related Peptide (ust2b) Gene as a Driver" @default.
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• W2028363596 cites W1930053815 @default.
• W2028363596 cites W1964214752 @default.
• W2028363596 cites W1967318866 @default.
• W2028363596 cites W1967906538 @default.
• W2028363596 cites W1974973994 @default.
• W2028363596 cites W1976391205 @default.
• W2028363596 cites W1978254752 @default.
• W2028363596 cites W1981239255 @default.
• W2028363596 cites W1984393899 @default.
• W2028363596 cites W1985148070 @default.
• W2028363596 cites W1985956243 @default.
• W2028363596 cites W1987239039 @default.
• W2028363596 cites W2001346650 @default.
• W2028363596 cites W2002015872 @default.
• W2028363596 cites W2004862584 @default.
• W2028363596 cites W2008205503 @default.
• W2028363596 cites W2009005402 @default.
• W2028363596 cites W2011186116 @default.
• W2028363596 cites W2013919550 @default.
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• W2028363596 cites W2020466261 @default.
• W2028363596 cites W2024321766 @default.
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• W2028363596 cites W2034175439 @default.
• W2028363596 cites W2037097175 @default.
• W2028363596 cites W2037947871 @default.
• W2028363596 cites W2041571283 @default.
• W2028363596 cites W2051546210 @default.
• W2028363596 cites W2051797386 @default.
• W2028363596 cites W2051845521 @default.
• W2028363596 cites W2051919953 @default.
• W2028363596 cites W2057119353 @default.
• W2028363596 cites W2057462857 @default.
• W2028363596 cites W2063512844 @default.
• W2028363596 cites W2066037819 @default.
• W2028363596 cites W2073060338 @default.
• W2028363596 cites W2076058637 @default.
• W2028363596 cites W2076574902 @default.
• W2028363596 cites W2079887214 @default.
• W2028363596 cites W2082311924 @default.
• W2028363596 cites W2083157093 @default.
• W2028363596 cites W2084845861 @default.
• W2028363596 cites W2090817549 @default.
• W2028363596 cites W2091136303 @default.
• W2028363596 cites W2103523795 @default.
• W2028363596 cites W2117723173 @default.
• W2028363596 cites W2123509007 @default.
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• W2028363596 cites W2167526106 @default.
• W2028363596 cites W2280956349 @default.
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• W2028363596 doi "https://doi.org/10.1371/journal.pone.0117370" @default.
• W2028363596 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4319907" @default.
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• W2028363596 hasPublicationYear "2015" @default.
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