Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028450304> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2028450304 endingPage "243" @default.
- W2028450304 startingPage "238" @default.
- W2028450304 abstract "The primary objective of this study was to examine the feasibility of using a mouse model to evaluate the immunogenicity of proteins as a potential method to determine occupational exposure guidelines. Mice were intratracheally administered a benchmark protein allergen, subtilisin Carlsberg (Alcalase) in detergent matrix once a week for 4 to 6 weeks and specific IgE and IgG1 levels were determined. In all experiments, specific IgE levels were determined by using a rat basophilic leukemia cell (RBL) release assay, while specific IgG1 was measured by an ELISA. A good correlation was observed between IgE titers determined by the RBL assay and rat passive cutaneous anaphylaxis assay. Intratracheal administration of protease with detergent matrix was found to result in significant IgE and IgG1 responses that were dose related. Detergent matrix was found to enhance the Alcalase-specific IgE and IgG1 response when administered by the intratracheal route. The IgG1 response was much more robust, easier to measure, and found to follow the IgE response. These results suggest that a mouse intratracheal model is a feasible approach to examining the immunogenic potency of enzymes using specific IgE or IgG1 as the end points. Additional development and validation of the mouse model with other types of proteins will be pursued." @default.
- W2028450304 created "2016-06-24" @default.
- W2028450304 creator A5058391493 @default.
- W2028450304 creator A5067797197 @default.
- W2028450304 creator A5088755152 @default.
- W2028450304 date "1996-01-01" @default.
- W2028450304 modified "2023-10-16" @default.
- W2028450304 title "Specific IgE and IgG1 Responses to Subtilisin Carlsberg (Alcalase) in Mice: Development of an Intratracheal Exposure Model" @default.
- W2028450304 cites W1539696212 @default.
- W2028450304 cites W1598136615 @default.
- W2028450304 cites W1599957556 @default.
- W2028450304 cites W1965614930 @default.
- W2028450304 cites W1967103023 @default.
- W2028450304 cites W1981793076 @default.
- W2028450304 cites W2017704828 @default.
- W2028450304 cites W2024123788 @default.
- W2028450304 cites W2062482845 @default.
- W2028450304 cites W2079893720 @default.
- W2028450304 cites W2113703610 @default.
- W2028450304 cites W2159987872 @default.
- W2028450304 cites W2169383488 @default.
- W2028450304 cites W2410568292 @default.
- W2028450304 doi "https://doi.org/10.1093/toxsci/29.2.238" @default.
- W2028450304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8742321" @default.
- W2028450304 hasPublicationYear "1996" @default.
- W2028450304 type Work @default.
- W2028450304 sameAs 2028450304 @default.
- W2028450304 citedByCount "14" @default.
- W2028450304 countsByYear W20284503042013 @default.
- W2028450304 countsByYear W20284503042015 @default.
- W2028450304 countsByYear W20284503042022 @default.
- W2028450304 crossrefType "journal-article" @default.
- W2028450304 hasAuthorship W2028450304A5058391493 @default.
- W2028450304 hasAuthorship W2028450304A5067797197 @default.
- W2028450304 hasAuthorship W2028450304A5088755152 @default.
- W2028450304 hasBestOaLocation W20284503041 @default.
- W2028450304 hasConcept C141105273 @default.
- W2028450304 hasConcept C147483822 @default.
- W2028450304 hasConcept C159654299 @default.
- W2028450304 hasConcept C181199279 @default.
- W2028450304 hasConcept C185592680 @default.
- W2028450304 hasConcept C202751555 @default.
- W2028450304 hasConcept C203014093 @default.
- W2028450304 hasConcept C2776714187 @default.
- W2028450304 hasConcept C2779811187 @default.
- W2028450304 hasConcept C2780868878 @default.
- W2028450304 hasConcept C55493867 @default.
- W2028450304 hasConcept C57992300 @default.
- W2028450304 hasConcept C71924100 @default.
- W2028450304 hasConcept C98274493 @default.
- W2028450304 hasConceptScore W2028450304C141105273 @default.
- W2028450304 hasConceptScore W2028450304C147483822 @default.
- W2028450304 hasConceptScore W2028450304C159654299 @default.
- W2028450304 hasConceptScore W2028450304C181199279 @default.
- W2028450304 hasConceptScore W2028450304C185592680 @default.
- W2028450304 hasConceptScore W2028450304C202751555 @default.
- W2028450304 hasConceptScore W2028450304C203014093 @default.
- W2028450304 hasConceptScore W2028450304C2776714187 @default.
- W2028450304 hasConceptScore W2028450304C2779811187 @default.
- W2028450304 hasConceptScore W2028450304C2780868878 @default.
- W2028450304 hasConceptScore W2028450304C55493867 @default.
- W2028450304 hasConceptScore W2028450304C57992300 @default.
- W2028450304 hasConceptScore W2028450304C71924100 @default.
- W2028450304 hasConceptScore W2028450304C98274493 @default.
- W2028450304 hasIssue "2" @default.
- W2028450304 hasLocation W20284503041 @default.
- W2028450304 hasLocation W20284503042 @default.
- W2028450304 hasOpenAccess W2028450304 @default.
- W2028450304 hasPrimaryLocation W20284503041 @default.
- W2028450304 hasRelatedWork W1995927784 @default.
- W2028450304 hasRelatedWork W2014990393 @default.
- W2028450304 hasRelatedWork W2024340173 @default.
- W2028450304 hasRelatedWork W2054881753 @default.
- W2028450304 hasRelatedWork W2084072824 @default.
- W2028450304 hasRelatedWork W2158369850 @default.
- W2028450304 hasRelatedWork W3106700065 @default.
- W2028450304 hasRelatedWork W4309786682 @default.
- W2028450304 hasRelatedWork W76695023 @default.
- W2028450304 hasRelatedWork W2187558299 @default.
- W2028450304 hasVolume "29" @default.
- W2028450304 isParatext "false" @default.
- W2028450304 isRetracted "false" @default.
- W2028450304 magId "2028450304" @default.
- W2028450304 workType "article" @default.