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• W2028455095 endingPage "497" @default.
• W2028455095 startingPage "481" @default.
• W2028455095 abstract "Dicamba (2-methoxy-3,6-dichlorobenzoic acid) O-demethylase (DMO) is the terminal Rieske oxygenase of a three-component system that includes a ferredoxin and a reductase. It catalyzes the NADH-dependent oxidative demethylation of the broad leaf herbicide dicamba. DMO represents the first crystal structure of a Rieske non-heme iron oxygenase that performs an exocyclic monooxygenation, incorporating O(2) into a side-chain moiety and not a ring system. The structure reveals a 3-fold symmetric trimer (alpha(3)) in the crystallographic asymmetric unit with similar arrangement of neighboring inter-subunit Rieske domain and non-heme iron site enabling electron transport consistent with other structurally characterized Rieske oxygenases. While the Rieske domain is similar, differences are observed in the catalytic domain, which is smaller in sequence length than those described previously, yet possessing an active-site cavity of larger volume when compared to oxygenases with larger substrates. Consistent with the amphipathic substrate, the active site is designed to interact with both the carboxylate and aromatic ring with both key polar and hydrophobic interactions observed. DMO structures were solved with and without substrate (dicamba), product (3,6-dichlorosalicylic acid), and either cobalt or iron in the non-heme iron site. The substitution of cobalt for iron revealed an uncommon mode of non-heme iron binding trapped by the non-catalytic Co(2+), which, we postulate, may be transiently present in the native enzyme during the catalytic cycle. Thus, we present four DMO structures with resolutions ranging from 1.95 to 2.2 A, which, in sum, provide a snapshot of a dynamic enzyme where metal binding and substrate binding are coupled to observed structural changes in the non-heme iron and catalytic sites." @default.
• W2028455095 created "2016-06-24" @default.
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• W2028455095 date "2009-09-01" @default.
• W2028455095 modified "2023-10-14" @default.
• W2028455095 title "Dicamba Monooxygenase: Structural Insights into a Dynamic Rieske Oxygenase that Catalyzes an Exocyclic Monooxygenation" @default.
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• W2028455095 cites W1539796472 @default.
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• W2028455095 cites W1748090422 @default.
• W2028455095 cites W1903986304 @default.
• W2028455095 cites W1965277349 @default.
• W2028455095 cites W1967700647 @default.
• W2028455095 cites W1968410649 @default.
• W2028455095 cites W1971336871 @default.
• W2028455095 cites W1976483743 @default.
• W2028455095 cites W1980152562 @default.
• W2028455095 cites W1983387636 @default.
• W2028455095 cites W1986191025 @default.
• W2028455095 cites W1988062176 @default.
• W2028455095 cites W1988949059 @default.
• W2028455095 cites W1991354940 @default.
• W2028455095 cites W1993398633 @default.
• W2028455095 cites W2000677225 @default.
• W2028455095 cites W2010464084 @default.
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• W2028455095 cites W2023689426 @default.
• W2028455095 cites W2023809773 @default.
• W2028455095 cites W2031648062 @default.
• W2028455095 cites W2036874089 @default.
• W2028455095 cites W2037253128 @default.
• W2028455095 cites W2042562529 @default.
• W2028455095 cites W2047423098 @default.
• W2028455095 cites W2050188620 @default.
• W2028455095 cites W2051066630 @default.
• W2028455095 cites W2051302441 @default.
• W2028455095 cites W2055611989 @default.
• W2028455095 cites W2056081749 @default.
• W2028455095 cites W2057222541 @default.
• W2028455095 cites W2058683340 @default.
• W2028455095 cites W2062577852 @default.
• W2028455095 cites W2084923757 @default.
• W2028455095 cites W2088335341 @default.
• W2028455095 cites W2095346321 @default.
• W2028455095 cites W2103794058 @default.
• W2028455095 cites W2106246537 @default.
• W2028455095 cites W2109515998 @default.
• W2028455095 cites W2110272427 @default.
• W2028455095 cites W2110352313 @default.
• W2028455095 cites W2110447523 @default.
• W2028455095 cites W2132879814 @default.
• W2028455095 cites W2138127241 @default.
• W2028455095 cites W2142464470 @default.
• W2028455095 cites W2143694829 @default.
• W2028455095 cites W2151107150 @default.
• W2028455095 cites W2152087460 @default.
• W2028455095 cites W2152877209 @default.
• W2028455095 cites W2158630348 @default.
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• W2028455095 cites W2163523836 @default.
• W2028455095 cites W2168064149 @default.
• W2028455095 cites W2168317596 @default.
• W2028455095 cites W2170434288 @default.
• W2028455095 cites W2338151994 @default.
• W2028455095 cites W2414296254 @default.
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• W2028455095 doi "https://doi.org/10.1016/j.jmb.2009.07.022" @default.
• W2028455095 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19616009" @default.
• W2028455095 hasPublicationYear "2009" @default.
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