Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028490079> ?p ?o ?g. }
Showing items 1 to 91 of
91
with 100 items per page.
- W2028490079 endingPage "18" @default.
- W2028490079 startingPage "15" @default.
- W2028490079 abstract "A sensitive and highly reproducible assay for N-acetylmuramoyl-l-alanine amidase (EC 3.5.1.28) was devised, based on specific and homogeneous l-[14C]alanine labeling of the substrate, the peptidoglycan. The method involves partial purification of both the enzyme and the substrate and monitoring the muropeptide cleavage by coupling fluorodinitrobenzene to freed l-alanine NH2 groups. After acid hydrolysis of the substrate, the resulting DNP-l-alanine and l-alanine are separated by TLC, and radioactive counts in relevant spots are determined. Application of the method to the autolysin-endowed strain and an autolysis-deficient flaD-bearing mutant has revealed (i) that the N-acetylmuramoyl-l-alanine amidase behaves like an endoenzyme with an apparent Kcat(s−1) of 40, and (ii) that the residual enzyme activity in the flaD bearing strain amounts to 2.5 (±0.1)% of that of the parent strain." @default.
- W2028490079 created "2016-06-24" @default.
- W2028490079 creator A5018788259 @default.
- W2028490079 creator A5022417306 @default.
- W2028490079 creator A5046900657 @default.
- W2028490079 date "1991-10-01" @default.
- W2028490079 modified "2023-09-25" @default.
- W2028490079 title "N-acetylmuramoyl-l-alanine amidase assay based on specific radioactive labeling of muropeptide l-alanine: Quantitation of the enzyme activity in the autolysin deficient Bacillus subtilis 168, flaD strain" @default.
- W2028490079 cites W1506252471 @default.
- W2028490079 cites W1545280196 @default.
- W2028490079 cites W1554639867 @default.
- W2028490079 cites W1719124555 @default.
- W2028490079 cites W1751732631 @default.
- W2028490079 cites W1856112995 @default.
- W2028490079 cites W1997979249 @default.
- W2028490079 cites W1998511438 @default.
- W2028490079 cites W2013240782 @default.
- W2028490079 cites W2046276994 @default.
- W2028490079 cites W2072251583 @default.
- W2028490079 cites W2106232933 @default.
- W2028490079 cites W2109136595 @default.
- W2028490079 cites W2118224417 @default.
- W2028490079 cites W2139597912 @default.
- W2028490079 cites W2170103578 @default.
- W2028490079 cites W4244774677 @default.
- W2028490079 doi "https://doi.org/10.1016/0003-2697(91)90499-j" @default.
- W2028490079 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1686374" @default.
- W2028490079 hasPublicationYear "1991" @default.
- W2028490079 type Work @default.
- W2028490079 sameAs 2028490079 @default.
- W2028490079 citedByCount "16" @default.
- W2028490079 countsByYear W20284900792013 @default.
- W2028490079 countsByYear W20284900792019 @default.
- W2028490079 crossrefType "journal-article" @default.
- W2028490079 hasAuthorship W2028490079A5018788259 @default.
- W2028490079 hasAuthorship W2028490079A5022417306 @default.
- W2028490079 hasAuthorship W2028490079A5046900657 @default.
- W2028490079 hasConcept C104810894 @default.
- W2028490079 hasConcept C181199279 @default.
- W2028490079 hasConcept C185592680 @default.
- W2028490079 hasConcept C18903297 @default.
- W2028490079 hasConcept C2776634448 @default.
- W2028490079 hasConcept C2776809874 @default.
- W2028490079 hasConcept C2777272437 @default.
- W2028490079 hasConcept C2777289219 @default.
- W2028490079 hasConcept C2779856020 @default.
- W2028490079 hasConcept C43617362 @default.
- W2028490079 hasConcept C515207424 @default.
- W2028490079 hasConcept C523546767 @default.
- W2028490079 hasConcept C54355233 @default.
- W2028490079 hasConcept C55493867 @default.
- W2028490079 hasConcept C65588867 @default.
- W2028490079 hasConcept C86803240 @default.
- W2028490079 hasConceptScore W2028490079C104810894 @default.
- W2028490079 hasConceptScore W2028490079C181199279 @default.
- W2028490079 hasConceptScore W2028490079C185592680 @default.
- W2028490079 hasConceptScore W2028490079C18903297 @default.
- W2028490079 hasConceptScore W2028490079C2776634448 @default.
- W2028490079 hasConceptScore W2028490079C2776809874 @default.
- W2028490079 hasConceptScore W2028490079C2777272437 @default.
- W2028490079 hasConceptScore W2028490079C2777289219 @default.
- W2028490079 hasConceptScore W2028490079C2779856020 @default.
- W2028490079 hasConceptScore W2028490079C43617362 @default.
- W2028490079 hasConceptScore W2028490079C515207424 @default.
- W2028490079 hasConceptScore W2028490079C523546767 @default.
- W2028490079 hasConceptScore W2028490079C54355233 @default.
- W2028490079 hasConceptScore W2028490079C55493867 @default.
- W2028490079 hasConceptScore W2028490079C65588867 @default.
- W2028490079 hasConceptScore W2028490079C86803240 @default.
- W2028490079 hasIssue "1" @default.
- W2028490079 hasLocation W20284900791 @default.
- W2028490079 hasLocation W20284900792 @default.
- W2028490079 hasOpenAccess W2028490079 @default.
- W2028490079 hasPrimaryLocation W20284900791 @default.
- W2028490079 hasRelatedWork W1529735378 @default.
- W2028490079 hasRelatedWork W1551485561 @default.
- W2028490079 hasRelatedWork W1562889353 @default.
- W2028490079 hasRelatedWork W1600548131 @default.
- W2028490079 hasRelatedWork W1651660036 @default.
- W2028490079 hasRelatedWork W1780415897 @default.
- W2028490079 hasRelatedWork W1883296241 @default.
- W2028490079 hasRelatedWork W2028490079 @default.
- W2028490079 hasRelatedWork W2107178561 @default.
- W2028490079 hasRelatedWork W4239872973 @default.
- W2028490079 hasVolume "198" @default.
- W2028490079 isParatext "false" @default.
- W2028490079 isRetracted "false" @default.
- W2028490079 magId "2028490079" @default.
- W2028490079 workType "article" @default.