FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028495589> ?p ?o ?g. }

• W2028495589 endingPage "1055" @default.
• W2028495589 startingPage "1035" @default.
• W2028495589 abstract "The goal of the present study was to isolate, for the first time, cytochrome oxidase subunit genes from murine brain complementary DNA library and to characterize the expression of these genes from mitochondrial and nuclear sources at both light and electron microscopic levels. Brain subunit III (mitochondrial) shared 100% identity with that of murine L cells. Subunit VIa (nuclear) was known to have tissue-specific isoforms in other species: the ubiquitous liver isoform and the heart/muscle isoform. Our brain subunit VIa shared 93% homology with that of the rat liver and 100% identity with the recently reported murine liver isoform, which is only 62% identical to that of the rat heart isoform. In situ hybridization with riboprobes revealed messenger RNA labelling that was similar, though not identical, to that of cytochrome oxidase histochemistry. Monocular enucleation in adult mice induced a significant down-regulation of both subunit messages in the contralateral lateral geniculate nucleus. However, the decrease in subunit III messenger RNAs surpassed that of subunit VIa at all time periods examined, suggesting that mitochondrial gene expression is more tightly regulated by neuronal activity than that of nuclear ones. At the electron microscopic level, subunit III messenger RNA was localized to the mitochondrial compartment in both cell bodies and processes, while that of nuclear-encoded subunit VIa was present exclusively in the extramitochondrial compartment of somata and not of dendrites or axons. Surprisingly, the message was primarily associated with the rough endoplasmic reticulum, suggesting a novel pathway for its synthesis and trafficking. Our results indicate that the unique properties of neurons impose special requirements for subunits of a single mitochondrial enzyme with dual genomic origins. At sites of high energy demands (such as postsynaptic dendrites and some axon terminals), mitochondrial-encoded cytochrome oxidase subunits can be locally transcribed and translated, and they provide the framework for the subsequent importation and incorporation of nuclear-encoded subunits, which are strictly synthesized in the cell bodies. Dynamic local energy needs are met when subunits from the two genomic sources are assembled to form functional holoenzymes." @default.
• W2028495589 created "2016-06-24" @default.
• W2028495589 creator A5002708015 @default.
• W2028495589 creator A5033554080 @default.
• W2028495589 creator A5045342454 @default.
• W2028495589 creator A5053646636 @default.
• W2028495589 date "1997-01-01" @default.
• W2028495589 modified "2023-10-03" @default.
• W2028495589 title "Brain cytochrome oxidase subunit complementary DNAs: isolation, subcloning, sequencing, light and electron microscopic in situ hybridization of transcripts, and regulation by neuronal activity" @default.
• W2028495589 cites W1044366479 @default.
• W2028495589 cites W110402765 @default.
• W2028495589 cites W126252753 @default.
• W2028495589 cites W1487728646 @default.
• W2028495589 cites W1575260930 @default.
• W2028495589 cites W1587675960 @default.
• W2028495589 cites W1590726325 @default.
• W2028495589 cites W1603367677 @default.
• W2028495589 cites W1614200591 @default.
• W2028495589 cites W1638263862 @default.
• W2028495589 cites W1680438645 @default.
• W2028495589 cites W1965486134 @default.
• W2028495589 cites W1967497524 @default.
• W2028495589 cites W1968746186 @default.
• W2028495589 cites W1968952472 @default.
• W2028495589 cites W1976901577 @default.
• W2028495589 cites W1978422552 @default.
• W2028495589 cites W1983570419 @default.
• W2028495589 cites W1989841259 @default.
• W2028495589 cites W1992767485 @default.
• W2028495589 cites W1995368565 @default.
• W2028495589 cites W1995956446 @default.
• W2028495589 cites W1999142381 @default.
• W2028495589 cites W2000471647 @default.
• W2028495589 cites W2004976934 @default.
• W2028495589 cites W2008019983 @default.
• W2028495589 cites W2009116733 @default.
• W2028495589 cites W2015282923 @default.
• W2028495589 cites W2017347785 @default.
• W2028495589 cites W2021167122 @default.
• W2028495589 cites W2024164720 @default.
• W2028495589 cites W2029130887 @default.
• W2028495589 cites W2035260257 @default.
• W2028495589 cites W2036065109 @default.
• W2028495589 cites W2040184291 @default.
• W2028495589 cites W2052647425 @default.
• W2028495589 cites W2056925842 @default.
• W2028495589 cites W2076097002 @default.
• W2028495589 cites W2077134026 @default.
• W2028495589 cites W2084422560 @default.
• W2028495589 cites W2100404198 @default.
• W2028495589 cites W2133556236 @default.
• W2028495589 cites W2134851967 @default.
• W2028495589 cites W2135792486 @default.
• W2028495589 cites W2138270253 @default.
• W2028495589 cites W2162079383 @default.
• W2028495589 cites W2164313207 @default.
• W2028495589 cites W2165268250 @default.
• W2028495589 cites W2176573833 @default.
• W2028495589 cites W2334283544 @default.
• W2028495589 cites W2399745737 @default.
• W2028495589 cites W243684803 @default.
• W2028495589 cites W3026794137 @default.
• W2028495589 cites W320886184 @default.
• W2028495589 cites W44824404 @default.
• W2028495589 doi "https://doi.org/10.1016/s0306-4522(96)00410-1" @default.
• W2028495589 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9027865" @default.
• W2028495589 hasPublicationYear "1997" @default.
• W2028495589 type Work @default.
• W2028495589 sameAs 2028495589 @default.
• W2028495589 citedByCount "40" @default.
• W2028495589 countsByYear W20284955892012 @default.
• W2028495589 countsByYear W20284955892019 @default.
• W2028495589 crossrefType "journal-article" @default.
• W2028495589 hasAuthorship W2028495589A5002708015 @default.
• W2028495589 hasAuthorship W2028495589A5033554080 @default.
• W2028495589 hasAuthorship W2028495589A5045342454 @default.
• W2028495589 hasAuthorship W2028495589A5053646636 @default.
• W2028495589 hasConcept C104292427 @default.
• W2028495589 hasConcept C104317684 @default.
• W2028495589 hasConcept C105580179 @default.
• W2028495589 hasConcept C123249941 @default.
• W2028495589 hasConcept C153911025 @default.
• W2028495589 hasConcept C28859421 @default.
• W2028495589 hasConcept C53345823 @default.
• W2028495589 hasConcept C55493867 @default.
• W2028495589 hasConcept C81439078 @default.
• W2028495589 hasConcept C86803240 @default.
• W2028495589 hasConcept C95444343 @default.
• W2028495589 hasConceptScore W2028495589C104292427 @default.
• W2028495589 hasConceptScore W2028495589C104317684 @default.
• W2028495589 hasConceptScore W2028495589C105580179 @default.
• W2028495589 hasConceptScore W2028495589C123249941 @default.
• W2028495589 hasConceptScore W2028495589C153911025 @default.
• W2028495589 hasConceptScore W2028495589C28859421 @default.
• W2028495589 hasConceptScore W2028495589C53345823 @default.
• W2028495589 hasConceptScore W2028495589C55493867 @default.
• W2028495589 hasConceptScore W2028495589C81439078 @default.
• W2028495589 hasConceptScore W2028495589C86803240 @default.

• next