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• W2028543816 abstract "RNA-based regulation and CRISPR/Cas transcription factors (CRISPR-TFs) have the potential to be integrated for the tunable modulation of gene networks. A major limitation of this methodology is that guide RNAs (gRNAs) for CRISPR-TFs can only be expressed from RNA polymerase III promoters in human cells, limiting their use for conditional gene regulation. We present new strategies that enable expression of functional gRNAs from RNA polymerase II promoters and multiplexed production of proteins and gRNAs from a single transcript in human cells. We use multiple RNA regulatory strategies, including RNA-triple-helix structures, introns, microRNAs, and ribozymes, with Cas9-based CRISPR-TFs and Cas6/Csy4-based RNA processing. Using these tools, we efficiently modulate endogenous promoters and implement tunable synthetic circuits, including multistage cascades and RNA-dependent networks that can be rewired with Csy4 to achieve complex behaviors. This toolkit can be used for programming scalable gene circuits and perturbing endogenous networks for biology, therapeutic, and synthetic biology applications." @default.
• W2028543816 created "2016-06-24" @default.
• W2028543816 creator A5002394616 @default.
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• W2028543816 creator A5037282404 @default.
• W2028543816 creator A5043650841 @default.
• W2028543816 creator A5060326269 @default.
• W2028543816 date "2014-05-01" @default.
• W2028543816 modified "2023-10-13" @default.
• W2028543816 title "Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells" @default.
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• W2028543816 doi "https://doi.org/10.1016/j.molcel.2014.04.022" @default.
• W2028543816 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4077618" @default.
• W2028543816 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24837679" @default.
• W2028543816 hasPublicationYear "2014" @default.
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