FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028586612> ?p ?o ?g. }

• W2028586612 endingPage "184" @default.
• W2028586612 startingPage "175" @default.
• W2028586612 abstract "Cyclic AMP-dependent protein kinase and 3H-cAMP-binding activities were determined in normal Balb 3T3 cells and compared with the same preparations from SV40, chemical, and spontaneous transformants of 3T3 cells. The cytosolic protein kinase activities and protein kinase activity ratios were similar in all cell lines, although when the normal 3T3 cytosol was prepared by homogenization it contained less 3H-cAMP binding activity than the transformed 3T3 cytosols. The Triton X-100 treated particulate fractions from the normal and transformed 3T3 cells contained similar protein kinase and binding activities. The isozymic profile of cAMP-dependent protein kinases was examined by DEAE-chromatography. The 3T3 cells contained only type II isozyme in either cytosolic or membrane fractions. All transformants of the 3T3 cells contained both type I and type II isozymes. Other cell cultures, including chicken embryo fibroblasts, rat kidney cells, and human or calf endothelial cells contained type I and type II isozymes. Binding of the photoaffinity analogue of cAMP, 8-N3 cAMP, to the regulatory subunits of protein kinases in sonicates obtained from Balb 3T3 and SV 3T3 cells followed by separation on SDS polyacrylamide electrophoresis showed that the amount of RII subunit was approximately equal in the two cell lines. RI in Balb 3T3 cells was detectable but in a much lower quantity than in SV 3T3 cells. The cyclic AMP dependent-protein kinases from Balb 3T3 cells appear to be different from SV 3T3 cells by three criteria: 3H-cAMP binding in homogenates, DEAE chromatographic separation of isozymes, and 8-N3 cAMP binding." @default.
• W2028586612 created "2016-06-24" @default.
• W2028586612 creator A5050300076 @default.
• W2028586612 creator A5078702117 @default.
• W2028586612 creator A5085283368 @default.
• W2028586612 creator A5087010408 @default.
• W2028586612 date "1981-08-01" @default.
• W2028586612 modified "2023-10-13" @default.
• W2028586612 title "Cyclic AMP-dependent protein kinases from Balb 3T3 cells and other 3T3 derived lines" @default.
• W2028586612 cites W133693420 @default.
• W2028586612 cites W1490352408 @default.
• W2028586612 cites W1555931521 @default.
• W2028586612 cites W1558592232 @default.
• W2028586612 cites W1570227839 @default.
• W2028586612 cites W1575451294 @default.
• W2028586612 cites W1680964876 @default.
• W2028586612 cites W1775749144 @default.
• W2028586612 cites W1970413801 @default.
• W2028586612 cites W1973361865 @default.
• W2028586612 cites W1976942562 @default.
• W2028586612 cites W1990776699 @default.
• W2028586612 cites W2000445419 @default.
• W2028586612 cites W2013216313 @default.
• W2028586612 cites W2014734061 @default.
• W2028586612 cites W2018723952 @default.
• W2028586612 cites W2034717845 @default.
• W2028586612 cites W2035292187 @default.
• W2028586612 cites W2038632400 @default.
• W2028586612 cites W2053432905 @default.
• W2028586612 cites W2053787906 @default.
• W2028586612 cites W2057456583 @default.
• W2028586612 cites W2061144747 @default.
• W2028586612 cites W2080307472 @default.
• W2028586612 cites W2091480744 @default.
• W2028586612 cites W2094115400 @default.
• W2028586612 cites W2100837269 @default.
• W2028586612 cites W2120331014 @default.
• W2028586612 cites W2141449078 @default.
• W2028586612 cites W346457056 @default.
• W2028586612 cites W37050816 @default.
• W2028586612 cites W65617761 @default.
• W2028586612 doi "https://doi.org/10.1002/jcp.1041080208" @default.
• W2028586612 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6267082" @default.
• W2028586612 hasPublicationYear "1981" @default.
• W2028586612 type Work @default.
• W2028586612 sameAs 2028586612 @default.
• W2028586612 citedByCount "25" @default.
• W2028586612 crossrefType "journal-article" @default.
• W2028586612 hasAuthorship W2028586612A5050300076 @default.
• W2028586612 hasAuthorship W2028586612A5078702117 @default.
• W2028586612 hasAuthorship W2028586612A5085283368 @default.
• W2028586612 hasAuthorship W2028586612A5087010408 @default.
• W2028586612 hasConcept C101660505 @default.
• W2028586612 hasConcept C104292427 @default.
• W2028586612 hasConcept C104317684 @default.
• W2028586612 hasConcept C119795356 @default.
• W2028586612 hasConcept C1491633281 @default.
• W2028586612 hasConcept C153911025 @default.
• W2028586612 hasConcept C179247698 @default.
• W2028586612 hasConcept C181199279 @default.
• W2028586612 hasConcept C184235292 @default.
• W2028586612 hasConcept C189014844 @default.
• W2028586612 hasConcept C2776746612 @default.
• W2028586612 hasConcept C54009773 @default.
• W2028586612 hasConcept C54355233 @default.
• W2028586612 hasConcept C55493867 @default.
• W2028586612 hasConcept C81885089 @default.
• W2028586612 hasConcept C86803240 @default.
• W2028586612 hasConcept C97029542 @default.
• W2028586612 hasConcept C98539663 @default.
• W2028586612 hasConceptScore W2028586612C101660505 @default.
• W2028586612 hasConceptScore W2028586612C104292427 @default.
• W2028586612 hasConceptScore W2028586612C104317684 @default.
• W2028586612 hasConceptScore W2028586612C119795356 @default.
• W2028586612 hasConceptScore W2028586612C1491633281 @default.
• W2028586612 hasConceptScore W2028586612C153911025 @default.
• W2028586612 hasConceptScore W2028586612C179247698 @default.
• W2028586612 hasConceptScore W2028586612C181199279 @default.
• W2028586612 hasConceptScore W2028586612C184235292 @default.
• W2028586612 hasConceptScore W2028586612C189014844 @default.
• W2028586612 hasConceptScore W2028586612C2776746612 @default.
• W2028586612 hasConceptScore W2028586612C54009773 @default.
• W2028586612 hasConceptScore W2028586612C54355233 @default.
• W2028586612 hasConceptScore W2028586612C55493867 @default.
• W2028586612 hasConceptScore W2028586612C81885089 @default.
• W2028586612 hasConceptScore W2028586612C86803240 @default.
• W2028586612 hasConceptScore W2028586612C97029542 @default.
• W2028586612 hasConceptScore W2028586612C98539663 @default.
• W2028586612 hasIssue "2" @default.
• W2028586612 hasLocation W20285866121 @default.
• W2028586612 hasLocation W20285866122 @default.
• W2028586612 hasOpenAccess W2028586612 @default.
• W2028586612 hasPrimaryLocation W20285866121 @default.
• W2028586612 hasRelatedWork W1550996414 @default.
• W2028586612 hasRelatedWork W1557025436 @default.
• W2028586612 hasRelatedWork W1763215782 @default.
• W2028586612 hasRelatedWork W1986393148 @default.
• W2028586612 hasRelatedWork W2009813704 @default.

• next