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- W2028589353 abstract "The cytosolic β-glucosidase of mammalian liver has been implicated in the metabolic transformation of plant glycosides, such as vicine and amygdalin, which are associated with the development of toxic syndromes. We investigated which cell types express cytosolic β-glucosidase in guinea pig liver, and characterized the contribution of this enzyme to the hydrolysis of aromatic glucosides in cultured cells and in tissue slices. Cytosolic β-glucosidase was expressed in hepatocytes and not in Kupffer or endothelial cells as determined by enzyme-specific activity and Western blots of liver cell extracts. Intracellular β-glucosidase activity was visualized using the fluorescent β-glucosidase substrate, resorufin β-d-glucoside, and shown to be caused by the cytosolic β-glucosidase using the inhibitors, conduritol β-epoxide and dinitrophenol-2-deoxy-2-fluoro-β-d-glucopyranoside (DNP2FGlc). Staining of fresh liver slices with resorufin β-glucoside revealed that cytosolic β-glucosidase is expressed in all hepatocytes, with no significant portal-central gradient. These data indicate that cytosolic β-glucosidase is a hepatocyte-specific enzyme, and support the hypothesis that cytosolic β-glucosidase in the liver functions to hydrolyze small glucosides absorbed by the intestine. Furthermore, toxic injury to cultured hepatocytes by CCl4 resulted in release of cytosolic β-glucosidase in parallel with the hepatocyte marker enzymes alanine transaminase and lactate dehydrogenase. This suggests that acute increases in serum levels of cytosolic β-glucosidase in animal models of liver injury may reflect direct injury of hepatocytes." @default.
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- W2028589353 date "1998-07-01" @default.
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- W2028589353 title "Expression of cytosolic ?-glucosidase in guinea pig liver cells" @default.
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- W2028589353 doi "https://doi.org/10.1002/hep.510280121" @default.
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