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- W2028707679 abstract "The homogeneity of pigeon liver fatty acid synthetase has been rigorously tested by physicochemical techniques and crossed-rocket immunoelectrophoresis. The enzyme has also been incubated for 1 h at 100 degrees C in 2% sodium dodecyl sulfate and 0.1 M dithiothreitol. The number of protein components on gel electrophoresis and of dansylated amino acids increased as a function of incubation time. Furthermore, the minor proteins observed after gel electrophoresis cross-reacted with antibody raised to the synthetase. Proteolysis was not chemically mediated by the detergent, the reducing agent or the buffer conditions chosen. Several commercially prepared proteins were not degraded by this procedure, and two proteins were recalcitrant to hydrolysis when included in the same incubation mixture as the synthetase. The inclusion of certain microbial proteinase inhibitors decreased the amount of degradation. This demonstrated that hydrolysis of the synthetase is mediated by a specific vertebrate enzyme which retains activity under denaturing conditions at 100 degrees C. Further degradation is also observed after individual treatment of four limited digestion products from the pigeon liver fatty acid synthetase, suggesting the possibility of an inherent proteolytic activity within the complex." @default.
- W2028707679 created "2016-06-24" @default.
- W2028707679 creator A5017506642 @default.
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- W2028707679 date "1983-04-01" @default.
- W2028707679 modified "2023-10-17" @default.
- W2028707679 title "Degradation of pigeon liver fatty acid synthetase in the absence of exogenous proteinases" @default.
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- W2028707679 doi "https://doi.org/10.1016/0167-4838(83)90343-6" @default.
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