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• W2028712739 abstract "Abstract We aimed to determine whether PAX 1/ SOX 1 methylation could be translated to clinical practice for cervical neoplasia detection when used alone and in combination with current cytology‐based Pap screening. We conducted a multicenter case–control study in 11 medical centers in Taiwan from December 2009 to November 2010. Six hundred seventy‐six patients were included in the analysis, including 330 in the training set and 346 in the testing set. Multiplex quantitative methylation‐specific polymerase chain reaction ( PCR ) was performed with a TaqMan probe system using a LightCycler 480 Real‐Time PCR System (Roche). The level of human papilloma virus ( HPV ) was analyzed using a Hybrid Capture 2 system (Digene). Receiver operating characteristic curves were generated to obtain the best cutoff values from the training data set. The sensitivities, specificities, and accuracies were validated in the testing set. The sensitivities for methylated ( m ) PAX 1 m and SOX 1 m and HPV testing for detecting CIN 3 + lesions were 0.64, 0.71, and 0.89, and the specificities were 0.91, 0.77, and 0.68, respectively. Combined parallel testing of PAX 1 m / SOX 1 m tests with Pap smearing showed superior specificity (0.84/0.71 vs. 0.66, respectively) and similar sensitivity (0.93/0.96 vs. 0.97) to the combination of Pap smear results and HPV testing. Thus, combined parallel testing using Pap smears and PAX 1 or SOX 1 methylation tests may provide better performance than a combination of Pap smears with HPV testing in detection for cervical neoplasia." @default.
• W2028712739 created "2016-06-24" @default.
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• W2028712739 date "2014-05-03" @default.
• W2028712739 modified "2023-10-16" @default.
• W2028712739 title "<i> <scp>PAX</scp> 1 </i> / <i> <scp>SOX</scp> 1 </i> <scp>DNA</scp> methylation and cervical neoplasia detection: a Taiwanese Gynecologic Oncology Group ( <scp>TGOG</scp> ) study" @default.
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• W2028712739 doi "https://doi.org/10.1002/cam4.253" @default.
• W2028712739 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4303175" @default.
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