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- W2028715067 abstract "Analytical electron microscopy provides high-resolution distributions of chemical elements by measuring characteristic core-edge signals that originate from interaction of the incident electrons with a thin section of a cell. Such elemental images give information about the organization of specific biomolecules within cellular organelles, as well as the distribution of ions involved in regulation of cellular processes. For example, mapping phosphorus enables visualization of nucleic acid, and calcium provides distributions of a major intracellular second messenger. Elemental mapping at ∼10 nm spatial resolution is typically achieved using energy-dispersive x-ray spectroscopy (EDXS) or electron energy loss spectroscopy (EELS) in a scanning transmission electron microscope (STEM). We have developed a complementary approach based on energy-filtered transmission electron microscopy (EFTEM). It is demonstrated that quantitative 2D elemental distributions containing ∼106 pixels can be obtained from large regions of cells, and that 3D elemental distributions can be obtained when EFTEM is combined with electron tomography. It is found that an accurate elemental distribution can be derived from just two energy-selected images, above and below a core-edge. However, since the core-edge signals for elements like calcium and phosphorus are relatively weak, it is important to model the spectral background carefully by correcting for plural scattering. We have applied quantitative ETFEM imaging and tomography to determine the 3D distributions of DNA in the cell nucleus, and to measure calcium in mitochondria of neurons." @default.
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- W2028715067 date "2009-02-01" @default.
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- W2028715067 title "New Approach To Quantitative Subcellular Imaging Of Phosphorus And Calcium Using Energy-filtered Transmission Electron Microscopy And Tomography" @default.
- W2028715067 doi "https://doi.org/10.1016/j.bpj.2008.12.3385" @default.
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