Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028751732> ?p ?o ?g. }
- W2028751732 endingPage "20" @default.
- W2028751732 startingPage "13" @default.
- W2028751732 abstract "Improvement of the biochemical characteristics of enzymes has been aided by misincorporation mutagenesis and DNA shuffling. Shuffling techniques can be used on a collection of mutants of the same gene, or related families of genes can be shuffled to produce mutants encoding chimeric gene products. One difficulty with current shuffling procedures is the predominance of unshuffled (‘parental’) molecules in the pool of mutants. We describe a procedure for gene shuffling using degenerate primers that allows control of the relative levels of recombination between the genes that are shuffled and reduces the regeneration of unshuffled parental genes. This procedure has the advantage of avoiding the use of endonucleases for gene fragmentation prior to shuffling and allows the use of random mutagenesis of selected segments of the gene as part of the procedure. We illustrate the use of the technique with a diverse family of β-xylanase genes that possess widely different G+C contents." @default.
- W2028751732 created "2016-06-24" @default.
- W2028751732 creator A5016482170 @default.
- W2028751732 creator A5027561103 @default.
- W2028751732 creator A5044873766 @default.
- W2028751732 date "2001-06-01" @default.
- W2028751732 modified "2023-10-09" @default.
- W2028751732 title "Degenerate oligonucleotide gene shuffling (DOGS): a method for enhancing the frequency of recombination with family shuffling" @default.
- W2028751732 cites W1639694146 @default.
- W2028751732 cites W1763997697 @default.
- W2028751732 cites W1807028851 @default.
- W2028751732 cites W190572224 @default.
- W2028751732 cites W1969126182 @default.
- W2028751732 cites W1992693020 @default.
- W2028751732 cites W1994116487 @default.
- W2028751732 cites W2001302607 @default.
- W2028751732 cites W2001307567 @default.
- W2028751732 cites W2011513747 @default.
- W2028751732 cites W2014345204 @default.
- W2028751732 cites W2020413336 @default.
- W2028751732 cites W2020720597 @default.
- W2028751732 cites W2028113659 @default.
- W2028751732 cites W2060579262 @default.
- W2028751732 cites W2060913542 @default.
- W2028751732 cites W2071086763 @default.
- W2028751732 cites W2087823077 @default.
- W2028751732 cites W2089650495 @default.
- W2028751732 cites W2099974092 @default.
- W2028751732 cites W2123030953 @default.
- W2028751732 cites W2124089608 @default.
- W2028751732 cites W2125811524 @default.
- W2028751732 cites W2147012166 @default.
- W2028751732 cites W2151900576 @default.
- W2028751732 cites W2165026145 @default.
- W2028751732 cites W2171972026 @default.
- W2028751732 cites W4248803889 @default.
- W2028751732 doi "https://doi.org/10.1016/s0378-1119(01)00506-6" @default.
- W2028751732 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11410361" @default.
- W2028751732 hasPublicationYear "2001" @default.
- W2028751732 type Work @default.
- W2028751732 sameAs 2028751732 @default.
- W2028751732 citedByCount "83" @default.
- W2028751732 countsByYear W20287517322012 @default.
- W2028751732 countsByYear W20287517322013 @default.
- W2028751732 countsByYear W20287517322014 @default.
- W2028751732 countsByYear W20287517322015 @default.
- W2028751732 countsByYear W20287517322016 @default.
- W2028751732 countsByYear W20287517322017 @default.
- W2028751732 countsByYear W20287517322018 @default.
- W2028751732 countsByYear W20287517322019 @default.
- W2028751732 countsByYear W20287517322020 @default.
- W2028751732 countsByYear W20287517322021 @default.
- W2028751732 countsByYear W20287517322022 @default.
- W2028751732 countsByYear W20287517322023 @default.
- W2028751732 crossrefType "journal-article" @default.
- W2028751732 hasAuthorship W2028751732A5016482170 @default.
- W2028751732 hasAuthorship W2028751732A5027561103 @default.
- W2028751732 hasAuthorship W2028751732A5044873766 @default.
- W2028751732 hasConcept C104317684 @default.
- W2028751732 hasConcept C129312508 @default.
- W2028751732 hasConcept C143065580 @default.
- W2028751732 hasConcept C156695909 @default.
- W2028751732 hasConcept C16318435 @default.
- W2028751732 hasConcept C167927819 @default.
- W2028751732 hasConcept C199360897 @default.
- W2028751732 hasConcept C2779454882 @default.
- W2028751732 hasConcept C41008148 @default.
- W2028751732 hasConcept C54355233 @default.
- W2028751732 hasConcept C70721500 @default.
- W2028751732 hasConcept C86803240 @default.
- W2028751732 hasConcept C9418097 @default.
- W2028751732 hasConceptScore W2028751732C104317684 @default.
- W2028751732 hasConceptScore W2028751732C129312508 @default.
- W2028751732 hasConceptScore W2028751732C143065580 @default.
- W2028751732 hasConceptScore W2028751732C156695909 @default.
- W2028751732 hasConceptScore W2028751732C16318435 @default.
- W2028751732 hasConceptScore W2028751732C167927819 @default.
- W2028751732 hasConceptScore W2028751732C199360897 @default.
- W2028751732 hasConceptScore W2028751732C2779454882 @default.
- W2028751732 hasConceptScore W2028751732C41008148 @default.
- W2028751732 hasConceptScore W2028751732C54355233 @default.
- W2028751732 hasConceptScore W2028751732C70721500 @default.
- W2028751732 hasConceptScore W2028751732C86803240 @default.
- W2028751732 hasConceptScore W2028751732C9418097 @default.
- W2028751732 hasIssue "1" @default.
- W2028751732 hasLocation W20287517321 @default.
- W2028751732 hasLocation W20287517322 @default.
- W2028751732 hasOpenAccess W2028751732 @default.
- W2028751732 hasPrimaryLocation W20287517321 @default.
- W2028751732 hasRelatedWork W1937520340 @default.
- W2028751732 hasRelatedWork W2060359405 @default.
- W2028751732 hasRelatedWork W2082005407 @default.
- W2028751732 hasRelatedWork W2378673801 @default.
- W2028751732 hasRelatedWork W2378985947 @default.
- W2028751732 hasRelatedWork W2392729240 @default.
- W2028751732 hasRelatedWork W2416136133 @default.
- W2028751732 hasRelatedWork W2468171055 @default.
- W2028751732 hasRelatedWork W2478713127 @default.