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- W2028754019 abstract "A strain of Aeromonas hydrophila producing copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate, abbreviated as PHBHHx, was successfully transformed by electroporation. The plasmid used was a broad host range plasmid pBBR1MCS. Electroporation conditions were varied systemically to develop an electroporation protocol. The optimal yield of transformant was approximately 4 × 102 CFU/μg DNA at 12.5 kV/cm and 1000 Ω, resulting in a time constant of approximately 5 ms. The A. hydrophila transformants expressed plasmid-encoded resistance to chloromphenicol. Plasmid DNA in the A. hydrophila transformant was stably maintained. This is the first report of transformation of bacteria A. hydrophila." @default.
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- W2028754019 date "2005-11-01" @default.
- W2028754019 modified "2023-10-16" @default.
- W2028754019 title "Electroporation-mediated transformation of Aeromonas hydrophila" @default.
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- W2028754019 doi "https://doi.org/10.1016/j.plasmid.2005.04.004" @default.
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