FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028756776> ?p ?o ?g. }

• W2028756776 endingPage "736" @default.
• W2028756776 startingPage "724" @default.
• W2028756776 abstract "Dynorphins are endogenous opioid peptide products of the prodynorphin gene. An extensive literature suggests that dynorphins have deleterious effects on CNS injury outcome. We thus examined whether a deficiency of dynorphin would protect against tissue damage after spinal cord injury (SCI), and if individual cell types would be specifically affected. Wild-type and prodynorphin−/− mice received a moderate contusion injury at 10th thoracic vertebrae (T10). Caspase-3 activity at the injury site was significantly decreased in tissue homogenates from prodynorphin−/− mice after 4 h. We examined frozen sections at 4 h post-injury by immunostaining for active caspase-3. At 3–4 mm rostral or caudal to the injury, >90% of all neurons, astrocytes and oligodendrocytes expressed active caspase-3 in both wild-type and knockout mice. At 6–7 mm, there were fewer caspase-3+ oligodendrocytes and astrocytes than at 3–4 mm. Importantly, caspase-3 activation was significantly lower in prodynorphin−/− oligodendrocytes and astrocytes, as compared with wild-type mice. In contrast, while caspase-3 expression in neurons also declined with further distance from the injury, there was no effect of genotype. Radioimmunoassay showed that dynorphin A(1-17) was regionally increased in wild-type injured versus sham-injured tissues, although levels of the prodynorphin processing product Arg6-Leu-enkephalin were unchanged. Our results indicate that dynorphin peptides affect the extent of post-injury caspase-3 activation, and that glia are especially sensitive to these effects. By promoting caspase-3 activation, dynorphin peptides likely increase the probability of glial apoptosis after SCI. While normally beneficial, our findings suggest that prodynorphin or its peptide products become maladaptive following SCI and contribute to secondary injury." @default.
• W2028756776 created "2016-06-24" @default.
• W2028756776 creator A5013943449 @default.
• W2028756776 creator A5018617711 @default.
• W2028756776 creator A5036342375 @default.
• W2028756776 creator A5039161354 @default.
• W2028756776 creator A5045907801 @default.
• W2028756776 creator A5058999301 @default.
• W2028756776 creator A5076458805 @default.
• W2028756776 date "2007-09-01" @default.
• W2028756776 modified "2023-10-17" @default.
• W2028756776 title "Caspase-3 activity is reduced after spinal cord injury in mice lacking dynorphin: Differential effects on glia and neurons" @default.
• W2028756776 cites W112514051 @default.
• W2028756776 cites W1433852924 @default.
• W2028756776 cites W1508419546 @default.
• W2028756776 cites W1530777667 @default.
• W2028756776 cites W1569061064 @default.
• W2028756776 cites W1573390290 @default.
• W2028756776 cites W1579810707 @default.
• W2028756776 cites W1709529434 @default.
• W2028756776 cites W187333225 @default.
• W2028756776 cites W1966086687 @default.
• W2028756776 cites W1966853012 @default.
• W2028756776 cites W1967544492 @default.
• W2028756776 cites W1967900234 @default.
• W2028756776 cites W1972292508 @default.
• W2028756776 cites W1977729873 @default.
• W2028756776 cites W1979196482 @default.
• W2028756776 cites W1980177820 @default.
• W2028756776 cites W1983289484 @default.
• W2028756776 cites W1984328054 @default.
• W2028756776 cites W1985243530 @default.
• W2028756776 cites W1985538179 @default.
• W2028756776 cites W1989033412 @default.
• W2028756776 cites W1990042480 @default.
• W2028756776 cites W1991982116 @default.
• W2028756776 cites W1998512503 @default.
• W2028756776 cites W1998722172 @default.
• W2028756776 cites W2008720843 @default.
• W2028756776 cites W2010460000 @default.
• W2028756776 cites W2012299945 @default.
• W2028756776 cites W2013877595 @default.
• W2028756776 cites W2015079240 @default.
• W2028756776 cites W2018544566 @default.
• W2028756776 cites W2020720142 @default.
• W2028756776 cites W2021540744 @default.
• W2028756776 cites W2023961353 @default.
• W2028756776 cites W2025297391 @default.
• W2028756776 cites W2026918087 @default.
• W2028756776 cites W2027295595 @default.
• W2028756776 cites W2027921840 @default.
• W2028756776 cites W2028991114 @default.
• W2028756776 cites W2031350131 @default.
• W2028756776 cites W2035274471 @default.
• W2028756776 cites W2037863091 @default.
• W2028756776 cites W2041284749 @default.
• W2028756776 cites W2045234723 @default.
• W2028756776 cites W2045375209 @default.
• W2028756776 cites W2045380850 @default.
• W2028756776 cites W2047889698 @default.
• W2028756776 cites W2051365317 @default.
• W2028756776 cites W2052487747 @default.
• W2028756776 cites W2053989901 @default.
• W2028756776 cites W2054912683 @default.
• W2028756776 cites W2055744694 @default.
• W2028756776 cites W2057723332 @default.
• W2028756776 cites W2059130466 @default.
• W2028756776 cites W2060081382 @default.
• W2028756776 cites W2062793260 @default.
• W2028756776 cites W2063019796 @default.
• W2028756776 cites W2067380183 @default.
• W2028756776 cites W2067680662 @default.
• W2028756776 cites W2067985632 @default.
• W2028756776 cites W2068127678 @default.
• W2028756776 cites W2069450754 @default.
• W2028756776 cites W2070254400 @default.
• W2028756776 cites W2070529844 @default.
• W2028756776 cites W2071833703 @default.
• W2028756776 cites W2072918771 @default.
• W2028756776 cites W2075588002 @default.
• W2028756776 cites W2081840105 @default.
• W2028756776 cites W2084625369 @default.
• W2028756776 cites W2084687782 @default.
• W2028756776 cites W2093076755 @default.
• W2028756776 cites W2094752791 @default.
• W2028756776 cites W2100290637 @default.
• W2028756776 cites W2103615857 @default.
• W2028756776 cites W2108803055 @default.
• W2028756776 cites W2132597602 @default.
• W2028756776 cites W2136019899 @default.
• W2028756776 cites W2137281156 @default.
• W2028756776 cites W2147337023 @default.
• W2028756776 cites W2152755443 @default.
• W2028756776 cites W2045818605 @default.
• W2028756776 doi "https://doi.org/10.1016/j.neuroscience.2007.05.053" @default.
• W2028756776 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17698296" @default.
• W2028756776 hasPublicationYear "2007" @default.
• W2028756776 type Work @default.

• next