Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028762492> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W2028762492 endingPage "509" @default.
- W2028762492 startingPage "505" @default.
- W2028762492 abstract "Rapid, nongenomic actions of steroid hormones have been characterized only recently. They may be mediated by interaction with a poorly characterized membrane receptor, by classic receptor located to the plasma membrane, or by interaction of the classic receptor with other signaling effectors. Among these, rapid effects of progesterone on human spermatozoa have been shown to be mediated by interaction with one or more membrane receptors. Two proteins, respectively of 57 and 28 kDa, representing the possible surface progesterone receptors in human spermatozoa, have been identified by our group employing an antibody (c-262) directed against the progesterone binding domain of the genomic receptor. The two proteins have been immunoprecipitated using c-262, isolated by 2D gel electrophoresis and analyzed by Maldi-Tof. Preliminary results of the analysis in data bank of the obtained masses suggest that the two proteins represent previously unidentified ones since they do not match with any protein in the database. We have also performed RT-PCR analysis with RNA extracted from human spermatozoa, utilizing various oligoprimers in different regions of the human progesterone genomic receptor. Results indicate the presence of transcripts for the complete genomic receptor. However, several previously published studies in the literature indicate the absence of expression of the genomic receptor in human spermatozoa. In this light posttranscriptional/posttraductional modifications of the receptor can be hypothesized. Interestingly, with primers amplifying in the DNA-binding domain of the progesterone receptor gene, we detected a higher molecular weight transcript when compared to the placenta. Further studies are needed to determine whether the sequences of the transcripts obtained by RT-PCR analysis of human sperm RNA match exactly with the human genomic receptor gene and to define the sequence of the higher molecular weight transcript detected in the DNA-binding region." @default.
- W2028762492 created "2016-06-24" @default.
- W2028762492 creator A5014662752 @default.
- W2028762492 creator A5016806501 @default.
- W2028762492 creator A5018791605 @default.
- W2028762492 creator A5070777080 @default.
- W2028762492 creator A5072038760 @default.
- W2028762492 creator A5077463507 @default.
- W2028762492 creator A5081731109 @default.
- W2028762492 date "2002-05-01" @default.
- W2028762492 modified "2023-09-23" @default.
- W2028762492 title "Characterization of membrane nongenomic receptors for progesterone in human spermatozoa" @default.
- W2028762492 cites W1528772866 @default.
- W2028762492 cites W1976103734 @default.
- W2028762492 cites W1992654871 @default.
- W2028762492 cites W2036911977 @default.
- W2028762492 cites W2047538251 @default.
- W2028762492 cites W2079154892 @default.
- W2028762492 cites W2108754858 @default.
- W2028762492 cites W2121275737 @default.
- W2028762492 cites W2132529855 @default.
- W2028762492 cites W2137756623 @default.
- W2028762492 cites W2289858573 @default.
- W2028762492 cites W2372928330 @default.
- W2028762492 cites W4230450772 @default.
- W2028762492 cites W4231621998 @default.
- W2028762492 cites W4237773305 @default.
- W2028762492 doi "https://doi.org/10.1016/s0039-128x(01)00173-8" @default.
- W2028762492 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11960628" @default.
- W2028762492 hasPublicationYear "2002" @default.
- W2028762492 type Work @default.
- W2028762492 sameAs 2028762492 @default.
- W2028762492 citedByCount "49" @default.
- W2028762492 countsByYear W20287624922012 @default.
- W2028762492 countsByYear W20287624922013 @default.
- W2028762492 countsByYear W20287624922014 @default.
- W2028762492 countsByYear W20287624922015 @default.
- W2028762492 countsByYear W20287624922017 @default.
- W2028762492 countsByYear W20287624922018 @default.
- W2028762492 crossrefType "journal-article" @default.
- W2028762492 hasAuthorship W2028762492A5014662752 @default.
- W2028762492 hasAuthorship W2028762492A5016806501 @default.
- W2028762492 hasAuthorship W2028762492A5018791605 @default.
- W2028762492 hasAuthorship W2028762492A5070777080 @default.
- W2028762492 hasAuthorship W2028762492A5072038760 @default.
- W2028762492 hasAuthorship W2028762492A5077463507 @default.
- W2028762492 hasAuthorship W2028762492A5081731109 @default.
- W2028762492 hasConcept C104317684 @default.
- W2028762492 hasConcept C121608353 @default.
- W2028762492 hasConcept C144647389 @default.
- W2028762492 hasConcept C153911025 @default.
- W2028762492 hasConcept C170493617 @default.
- W2028762492 hasConcept C197462201 @default.
- W2028762492 hasConcept C41625074 @default.
- W2028762492 hasConcept C530470458 @default.
- W2028762492 hasConcept C54355233 @default.
- W2028762492 hasConcept C55493867 @default.
- W2028762492 hasConcept C71829478 @default.
- W2028762492 hasConcept C84606932 @default.
- W2028762492 hasConcept C86803240 @default.
- W2028762492 hasConcept C95444343 @default.
- W2028762492 hasConcept C98717036 @default.
- W2028762492 hasConceptScore W2028762492C104317684 @default.
- W2028762492 hasConceptScore W2028762492C121608353 @default.
- W2028762492 hasConceptScore W2028762492C144647389 @default.
- W2028762492 hasConceptScore W2028762492C153911025 @default.
- W2028762492 hasConceptScore W2028762492C170493617 @default.
- W2028762492 hasConceptScore W2028762492C197462201 @default.
- W2028762492 hasConceptScore W2028762492C41625074 @default.
- W2028762492 hasConceptScore W2028762492C530470458 @default.
- W2028762492 hasConceptScore W2028762492C54355233 @default.
- W2028762492 hasConceptScore W2028762492C55493867 @default.
- W2028762492 hasConceptScore W2028762492C71829478 @default.
- W2028762492 hasConceptScore W2028762492C84606932 @default.
- W2028762492 hasConceptScore W2028762492C86803240 @default.
- W2028762492 hasConceptScore W2028762492C95444343 @default.
- W2028762492 hasConceptScore W2028762492C98717036 @default.
- W2028762492 hasIssue "6" @default.
- W2028762492 hasLocation W20287624921 @default.
- W2028762492 hasLocation W20287624922 @default.
- W2028762492 hasOpenAccess W2028762492 @default.
- W2028762492 hasPrimaryLocation W20287624921 @default.
- W2028762492 hasRelatedWork W1498968793 @default.
- W2028762492 hasRelatedWork W2008994330 @default.
- W2028762492 hasRelatedWork W2028762492 @default.
- W2028762492 hasRelatedWork W2036110122 @default.
- W2028762492 hasRelatedWork W2050316456 @default.
- W2028762492 hasRelatedWork W2078422320 @default.
- W2028762492 hasRelatedWork W2142925367 @default.
- W2028762492 hasRelatedWork W2402117251 @default.
- W2028762492 hasRelatedWork W2410355257 @default.
- W2028762492 hasRelatedWork W4252457075 @default.
- W2028762492 hasVolume "67" @default.
- W2028762492 isParatext "false" @default.
- W2028762492 isRetracted "false" @default.
- W2028762492 magId "2028762492" @default.
- W2028762492 workType "article" @default.