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- W2028830780 abstract "We report the use of ultra performance liquid chromatography (UPLC) separation together with evaporative light scattering detection (ELSD) to effectively distinguish honey adulterated with maltooligosaccharides. UPLC separation utilizing a mobile phase with pure water and acetonitrile containing 0.2% triethylamine, resulted in enhanced separation and a running time of less than 6 min. Also, only a simple liquid–liquid extraction was required for the sample pretreatment. This UPLC–ELSD method provides limits of quantifications (LOQs) from 0.30 to 0.60 mg/g for the six maltooligosaccharides (maltose, maltotriose, maltotetraose, maltopentaose, maltohexaose and maltoheptaose) ranging from 15 to 2000 μg/mL with excellent linearity(R2 ≥ 0.9914). This optimized method also offers a satisfactory intra-day precision of 2.4%–9.4%, inter-day precision of 5.5%–12.1% and accuracy of − 7.7%–+ 11.4% at each level. We successfully applied this system for detecting maltooligosaccharide contamination in 290 honey samples collected from apiaries, supermarkets and traditional agri-product markets. Concentrations of the six maltooligosaccharides varied from undetectable to 14.1 mg/mL. The lowest quantifiable concentration that could be detected by this established method was 0.132 mg/mL. This study therefore demonstrates the reliability and potential use of the UPLC–ELSD technique in scrutinizing the authenticity of honey in the food processing industry." @default.
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- W2028830780 date "2014-02-01" @default.
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- W2028830780 title "Analysis of maltooligosaccharides in honey samples by ultra-performance liquid chromatography coupled with evaporative light scattering detection" @default.
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- W2028830780 doi "https://doi.org/10.1016/j.foodres.2014.01.014" @default.
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