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- W2028849145 abstract "The in vitro regeneration of Fraxinus angustifolia through bud/shoot organogenesis on embryonic explants was investigated. Embryo axes and cotyledons from mature seeds underwent adventitious regeneration after exposure to growth regulators, the embryo axes showing a greater regenerative potential than cotyledons. While the MS medium proved effective for the induction and initiation phases, it was inadequate for bud development, and was replaced by DKW medium. A constant supply of benzyladenine (BA) appeared essential for all phases. The gelling agents used had an important role in morphogenesis: Duchefa Gelrite positively affected the number of new buds per explant. The rooting of developed shoots was easily achieved on auxin-free WPM medium and most of the rooted plantlets “hardened” after a period of acclimation in a mist greenhouse." @default.
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- W2028849145 date "2001-03-01" @default.
- W2028849145 modified "2023-10-17" @default.
- W2028849145 title "Plant regeneration of Fraxinus angustifolia by in vitro shoot organogenesis" @default.
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- W2028849145 doi "https://doi.org/10.1016/s0304-4238(00)00178-3" @default.
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