Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028854536> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2028854536 endingPage "615" @default.
- W2028854536 startingPage "611" @default.
- W2028854536 abstract "Interaction between proteins (as analytes) and de novo designed structured peptides as capture molecules cause structural changes, which are reflected in fluorescent-intensity changes of labeled peptides in a dose dependent manner. In contrast to conventional detection methods our detection system does not involve the detection of specific molecules themselves in a 1:1 manner, but uses the principle of the differences in fluorescent intensity changes of capture peptides upon addition of analytes. Instead of the use of secondary antibodies we have attempted monitoring these structural changes by an array of de novo designed synthetic and structured peptides. In the present study we have focused on a recognition system, 5-fluorouracil, as a low molecular antigen and a monoclonal antibody against 5-FU. The fluorescent intensity changes of fluorescent labeled peptides have been measured after incubation with a monoclonal antibody and again after further incubation with the antigen, 5-FU. Unique intensity changes were found for several peptides in the fluorescent peptide library that allowed the visualization as a color-coded protein fingerprint. The peptide screen used in the present study offers a useful detection system as capture molecules for peptide-based microarrays." @default.
- W2028854536 created "2016-06-24" @default.
- W2028854536 creator A5025452150 @default.
- W2028854536 creator A5029444784 @default.
- W2028854536 creator A5043860090 @default.
- W2028854536 date "2015-02-01" @default.
- W2028854536 modified "2023-09-27" @default.
- W2028854536 title "Recognition of a monoclonal antibody against a small molecular weight antigen by monitoring the antigen–antibody reaction using fluorescence labeled structured peptides" @default.
- W2028854536 cites W109577459 @default.
- W2028854536 cites W1562815879 @default.
- W2028854536 cites W1970023472 @default.
- W2028854536 cites W1971372541 @default.
- W2028854536 cites W1977851761 @default.
- W2028854536 cites W1987881523 @default.
- W2028854536 cites W2020451776 @default.
- W2028854536 cites W2021888547 @default.
- W2028854536 cites W2034993353 @default.
- W2028854536 cites W2044310581 @default.
- W2028854536 cites W2070470216 @default.
- W2028854536 cites W2079737565 @default.
- W2028854536 cites W2087014867 @default.
- W2028854536 cites W2120810198 @default.
- W2028854536 cites W2317976731 @default.
- W2028854536 cites W2413745414 @default.
- W2028854536 cites W70790455 @default.
- W2028854536 doi "https://doi.org/10.1016/j.bmcl.2014.12.009" @default.
- W2028854536 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25541386" @default.
- W2028854536 hasPublicationYear "2015" @default.
- W2028854536 type Work @default.
- W2028854536 sameAs 2028854536 @default.
- W2028854536 citedByCount "3" @default.
- W2028854536 countsByYear W20288545362018 @default.
- W2028854536 countsByYear W20288545362021 @default.
- W2028854536 countsByYear W20288545362023 @default.
- W2028854536 crossrefType "journal-article" @default.
- W2028854536 hasAuthorship W2028854536A5025452150 @default.
- W2028854536 hasAuthorship W2028854536A5029444784 @default.
- W2028854536 hasAuthorship W2028854536A5043860090 @default.
- W2028854536 hasConcept C10390740 @default.
- W2028854536 hasConcept C121332964 @default.
- W2028854536 hasConcept C147483822 @default.
- W2028854536 hasConcept C153911025 @default.
- W2028854536 hasConcept C159654299 @default.
- W2028854536 hasConcept C185592680 @default.
- W2028854536 hasConcept C195616568 @default.
- W2028854536 hasConcept C203014093 @default.
- W2028854536 hasConcept C2779281246 @default.
- W2028854536 hasConcept C43617362 @default.
- W2028854536 hasConcept C542903549 @default.
- W2028854536 hasConcept C55493867 @default.
- W2028854536 hasConcept C62520636 @default.
- W2028854536 hasConcept C86803240 @default.
- W2028854536 hasConcept C91881484 @default.
- W2028854536 hasConceptScore W2028854536C10390740 @default.
- W2028854536 hasConceptScore W2028854536C121332964 @default.
- W2028854536 hasConceptScore W2028854536C147483822 @default.
- W2028854536 hasConceptScore W2028854536C153911025 @default.
- W2028854536 hasConceptScore W2028854536C159654299 @default.
- W2028854536 hasConceptScore W2028854536C185592680 @default.
- W2028854536 hasConceptScore W2028854536C195616568 @default.
- W2028854536 hasConceptScore W2028854536C203014093 @default.
- W2028854536 hasConceptScore W2028854536C2779281246 @default.
- W2028854536 hasConceptScore W2028854536C43617362 @default.
- W2028854536 hasConceptScore W2028854536C542903549 @default.
- W2028854536 hasConceptScore W2028854536C55493867 @default.
- W2028854536 hasConceptScore W2028854536C62520636 @default.
- W2028854536 hasConceptScore W2028854536C86803240 @default.
- W2028854536 hasConceptScore W2028854536C91881484 @default.
- W2028854536 hasIssue "3" @default.
- W2028854536 hasLocation W20288545361 @default.
- W2028854536 hasLocation W20288545362 @default.
- W2028854536 hasOpenAccess W2028854536 @default.
- W2028854536 hasPrimaryLocation W20288545361 @default.
- W2028854536 hasRelatedWork W1604248381 @default.
- W2028854536 hasRelatedWork W1973692620 @default.
- W2028854536 hasRelatedWork W1989765228 @default.
- W2028854536 hasRelatedWork W1996575012 @default.
- W2028854536 hasRelatedWork W1999614011 @default.
- W2028854536 hasRelatedWork W2001280522 @default.
- W2028854536 hasRelatedWork W2059923545 @default.
- W2028854536 hasRelatedWork W2162389703 @default.
- W2028854536 hasRelatedWork W2400620861 @default.
- W2028854536 hasRelatedWork W4239344034 @default.
- W2028854536 hasVolume "25" @default.
- W2028854536 isParatext "false" @default.
- W2028854536 isRetracted "false" @default.
- W2028854536 magId "2028854536" @default.
- W2028854536 workType "article" @default.