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- W2028856687 abstract "Abstract 3H-Labelled berberine, a quaternary benzylisoquinoline alkaloid, was shown to bind specifically to a microsomal fraction prepared from cultured cells of Coptis japonica capable of accumulating the alkaloid in the vacuole. The Scatchard plot of the data suggested the existence of a single berberine-binding site with a dissociation constant (Kd) of 1.67 μM for berberine. The binding of [3H]berberine to the membrane fraction was reversible, the optimum pH for binding being between 9 and 10. An addition of either coptisine or jatrorrhizine, which are congeners of berberine, to the assay mixture significantly decreased [3H] berberine-binding, indicating that the binding is highly specific for protoberberine-type alkaloids indigenous to the C. japonica. An SH-blocking reagent, iodoacetate, as well as an excess amount of inorganic cation almost completely inhibited the binding. Further fractionation of the microsomes by sucrose density-gradient centrifugation suggested that the binding site was not associated with plasma membrane, endoplasmic reticulum or tonoplast, but with an unidentified membrane (ϱ = 1.08–1.10 g cm−3), which might be involved in the transport of berberine to the vacuole." @default.
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- W2028856687 date "1994-08-01" @default.
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- W2028856687 title "Binding of berberine to a membrane fraction from Coptis cells" @default.
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- W2028856687 doi "https://doi.org/10.1016/s0031-9422(00)89725-3" @default.
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