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• W2028866485 abstract "Objective: Fluorescence is widely investigated and can characterize general changes occurring in the state of the cell and the tissue during physiological and/or pathological processes, but the method has not been adopted for clinical use. We present a photodiagnostic device and a relevant evaluation method fitted to classical screening procedures. This method is based on the discovery of smear content intrinsic fluorescent markers. Background data: Meaningful spectral components of cervical smear samples differ from those measured in the entire live cervix. This article deals with the identification of changes in smear spectra in cervicitis and CIN2+ (cervical intraepithelial neoplasia of the second degree or higher) at 355 nm excitation. Methods: Methods used in the study: microlaser-induced fluorescence spectroscopy of liquid cytology samples and histological evaluation of the biopsies of the same cervix (and/or only cytological evaluation) was performed for 78 cases. The fluorescence spectra of cervical cytology supernatant sediment were approximated with several Gaussian components. The ratios of the area under each Gaussian component to the whole area under the experimental curve were calculated and compared among histological groups by using the Mann–Whitney test and receiver operating characteristic (ROC) analysis. Results: The results of this study are a concise summary of the essential features verified by the data: the spectral regions 402–416 nm and 424–438 nm are important for the discrimination of both normal and CIN2+ groups (in terms of sensitivity, specificity, and positive predictive value). The spectral regions 480–515 nm and 595–625 nm are important for the identification of cervicitis. Conclusions: Cervical smear autofluorescence diagnostics could be useful for cancer screening at the point of care, in a simple cytology laboratory, and for the monitoring of treatment. We suggest possible fluorophores in the smear content." @default.
• W2028866485 created "2016-06-24" @default.
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• W2028866485 date "2012-05-01" @default.
• W2028866485 modified "2023-09-26" @default.
• W2028866485 title "Cervical Smear Photodiagnosis by Fluorescence" @default.
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• W2028866485 doi "https://doi.org/10.1089/pho.2011.3092" @default.
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