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- W2028876859 abstract "tsJT60, a temperature-sensitive (ts) cell-cycle mutant of Fischer rats, is viable at both the permissive (34 °C) and nonpermissive (40 °C) temperatures. The cells grow normally in exponential growth phase at both temperatures, but when stimulated with serum from G0 phase they enter S phase at 34 °C but not at 40 °C. tsJT60 cells transformed with human adenovirus (Ad) 12 dl205, which lacks the E1B 19-kDa polypeptide gene, were lethal at 40 °C, whereas tsJT60 cells transformed with Ad12 wt, dl207, which lacks E1B 58-kDa protein gene, or in206B, which produces 19- to 58- kDa fused protein, were viable. Degradation of cell DNA occurred in dl205-transformed tsJT60 cultured at both 34 °C and 40 °C. Neither cytocidal phenotype nor degradation of DNA occurred in 3Y1cells (a parental line of tsJT60) transformed with dl205. These results suggest that the lethal phenotype and degradation of DNA are related to the ts mutation in tsJT60 and also to the lack of Ad12 E1B 19kDa polypeptide." @default.
- W2028876859 created "2016-06-24" @default.
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- W2028876859 date "1988-11-01" @default.
- W2028876859 modified "2023-10-18" @default.
- W2028876859 title "A cell cycle G0-ts mutant, tsJT60, becomes lethal at the nonpermissive temperature after transformation with adenovirus 12 E1B 19K mutant" @default.
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- W2028876859 doi "https://doi.org/10.1016/0014-4827(88)90347-3" @default.
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