Matches in SemOpenAlex for { <https://semopenalex.org/work/W2028878613> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2028878613 endingPage "333" @default.
- W2028878613 startingPage "329" @default.
- W2028878613 abstract "Amino acid limitation leads in E. coli relA cells which cannot synthesize guanosine tetraphosphate (ppGpp) under these conditions to an amplification of pBR 322 DNA. We previously proposed that ppGpp produced in E. coli relA+ cells subjected to amino acid limitation inhibits pBR 322 DNA replication (Hecker et al. 1983). In further experiments it was established that an E. coli relA strain shows plasmid amplification during amino acid limitation (arginine, threonine, leucine or histidine) only in the presence of sufficient concentrations of phosphate, ammonia and glucose. Plasmid amplification does not occur if ammonia or phosphate is depleted. We suggest that glucose, ammonia and phosphate are needed for nucleotide and deoxynucleotide synthesis as the essential prerequisite for plasmid amplification. The activity of beta-lactamase was determined as an indicator for the expression of plasmid-encoded genes. The enzyme activity remains on a low level during plasmid amplification because of arginine exhaustion. A remarkable increase in the activity of beta-lactamase was observed after resumption of growth of relA cells containing amplified plasmid DNA. The plasmid content decreased as the cells continued to grow. We found that plasmid amplification and expression of plasmid-localized genes are opposite reactions which do not occur at the same time." @default.
- W2028878613 created "2016-06-24" @default.
- W2028878613 creator A5009508331 @default.
- W2028878613 creator A5065644581 @default.
- W2028878613 creator A5082748007 @default.
- W2028878613 date "1986-01-01" @default.
- W2028878613 modified "2023-09-26" @default.
- W2028878613 title "Physiological studies on pBR 322 DNA amplification in anEscherichia coli relA strain" @default.
- W2028878613 cites W1634834580 @default.
- W2028878613 cites W1858120728 @default.
- W2028878613 cites W1890358491 @default.
- W2028878613 cites W1974129985 @default.
- W2028878613 cites W1997339974 @default.
- W2028878613 cites W2018267779 @default.
- W2028878613 cites W2082043912 @default.
- W2028878613 cites W2089386865 @default.
- W2028878613 cites W2108968632 @default.
- W2028878613 cites W2111390925 @default.
- W2028878613 cites W2134055885 @default.
- W2028878613 cites W2412557235 @default.
- W2028878613 cites W2605972783 @default.
- W2028878613 cites W3190443527 @default.
- W2028878613 doi "https://doi.org/10.1002/jobm.3620260605" @default.
- W2028878613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3543293" @default.
- W2028878613 hasPublicationYear "1986" @default.
- W2028878613 type Work @default.
- W2028878613 sameAs 2028878613 @default.
- W2028878613 citedByCount "6" @default.
- W2028878613 crossrefType "journal-article" @default.
- W2028878613 hasAuthorship W2028878613A5009508331 @default.
- W2028878613 hasAuthorship W2028878613A5065644581 @default.
- W2028878613 hasAuthorship W2028878613A5082748007 @default.
- W2028878613 hasConcept C104317684 @default.
- W2028878613 hasConcept C105702510 @default.
- W2028878613 hasConcept C109932267 @default.
- W2028878613 hasConcept C153911025 @default.
- W2028878613 hasConcept C205225487 @default.
- W2028878613 hasConcept C22744801 @default.
- W2028878613 hasConcept C26628138 @default.
- W2028878613 hasConcept C2778022156 @default.
- W2028878613 hasConcept C515207424 @default.
- W2028878613 hasConcept C547475151 @default.
- W2028878613 hasConcept C552990157 @default.
- W2028878613 hasConcept C55493867 @default.
- W2028878613 hasConcept C86803240 @default.
- W2028878613 hasConceptScore W2028878613C104317684 @default.
- W2028878613 hasConceptScore W2028878613C105702510 @default.
- W2028878613 hasConceptScore W2028878613C109932267 @default.
- W2028878613 hasConceptScore W2028878613C153911025 @default.
- W2028878613 hasConceptScore W2028878613C205225487 @default.
- W2028878613 hasConceptScore W2028878613C22744801 @default.
- W2028878613 hasConceptScore W2028878613C26628138 @default.
- W2028878613 hasConceptScore W2028878613C2778022156 @default.
- W2028878613 hasConceptScore W2028878613C515207424 @default.
- W2028878613 hasConceptScore W2028878613C547475151 @default.
- W2028878613 hasConceptScore W2028878613C552990157 @default.
- W2028878613 hasConceptScore W2028878613C55493867 @default.
- W2028878613 hasConceptScore W2028878613C86803240 @default.
- W2028878613 hasIssue "6" @default.
- W2028878613 hasLocation W20288786131 @default.
- W2028878613 hasLocation W20288786132 @default.
- W2028878613 hasOpenAccess W2028878613 @default.
- W2028878613 hasPrimaryLocation W20288786131 @default.
- W2028878613 hasRelatedWork W1969249387 @default.
- W2028878613 hasRelatedWork W1981289100 @default.
- W2028878613 hasRelatedWork W1993000734 @default.
- W2028878613 hasRelatedWork W2012496869 @default.
- W2028878613 hasRelatedWork W2041480346 @default.
- W2028878613 hasRelatedWork W2054464810 @default.
- W2028878613 hasRelatedWork W2061281406 @default.
- W2028878613 hasRelatedWork W2297540085 @default.
- W2028878613 hasRelatedWork W2403499680 @default.
- W2028878613 hasRelatedWork W3023663517 @default.
- W2028878613 hasVolume "26" @default.
- W2028878613 isParatext "false" @default.
- W2028878613 isRetracted "false" @default.
- W2028878613 magId "2028878613" @default.
- W2028878613 workType "article" @default.