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- W2028899567 abstract "A high-performance liquid chromatographic (HPLC) method was developed and validated for the determination of busulfan in plasma. Busulfan was extracted in toluene, derivatized by 2,3,5,6-tetrafluorothiophenol to obtain di-TFTP-butane, the derivatization product was then re-extracted in toluene and injected into the HPLC system with ultraviolet detection (wavelength: 275 nm). Recovery from extraction was 80%, the limit of quantification was 50 ng/ml and linearity ranged from 50 to 2000 ng/ml. In addition, forty-two samples obtained from pediatric patients treated with busulfan were analyzed by the HPLC and GC-MS assays based on the same derivatization procedure. The correlation between the di-TFTP-butane concentrations was highly significant (p<0.0001), demonstrating that the two methods were in good agreement." @default.
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- W2028899567 date "1999-01-01" @default.
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- W2028899567 title "High-performance liquid chromatographic method for quantification of busulfan in plasma after derivatization by tetrafluorothiophenol" @default.
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- W2028899567 doi "https://doi.org/10.1016/s0378-4347(98)00473-3" @default.
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