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- W2028929738 abstract "A radioimmunoassay for the natural plant growth regulator (−)-jasmonic acid was established. The polyclonal antiserum was raised in rabbits against (−)-JA linked to hemocyanine. For tracer synthesis (−)-JA was conjugated to tyrosine ethyl ester and the 4-hydroxyphenyl moiety of this helper molecule labelled at 3-position by 125iodine. Crossreactivity studies showed the antiserum to be specific for (−)-JA and in a reduced manner to (+)-7-iso-jasmonic acid, accompanying (−)-JAin natural and synthetic preparations. The RIA has a detection limit of 0.8 ng (−)-JA-Me, the measuring range extends from 1 to 70 ng (−)-JA-Me, and coefficients of variation for triplicate standards were in the range of 1–4%. ln JA analysis the RIA described is superior to GC, HPLC, and bioassay with regard to sensitivity, low extract purification and small amount of plant material to be analyzed." @default.
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- W2028929738 date "1990-01-01" @default.
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- W2028929738 title "Radioimmunoassay for the Natural Plant Growth Regulator (−)-Jasmonic Acid" @default.
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- W2028929738 doi "https://doi.org/10.1016/s0015-3796(11)80239-8" @default.
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