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- W2028930006 abstract "We have studied the feasibility of purifying rat C6 glioma plasma membranes by a phase partitioning approach. The purification procedure involves cell homogenization and fractionation with an aqueous two-phase polymer system followed by selective affinity purification of plasma membranes by a wheat germ agglutinin-coupled polymer system. We demonstrate that the two-phase affinity partitioning technique is a simple and efficient method of isolating cell plasma membranes with high purity and yield. Furthermore, the isolated plasma membranes retain their functional integrity, as shown by the high-affinity insulin-like growth factor-I (IGF-I) binding capacity of IGF-I receptors." @default.
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- W2028930006 date "2000-01-01" @default.
- W2028930006 modified "2023-09-23" @default.
- W2028930006 title "Purification of Rat C6 Glioma Plasma Membranes by Affinity Partitioning" @default.
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- W2028930006 doi "https://doi.org/10.1159/000014622" @default.
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