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• W2028931781 abstract "Oocytes have been successfully cryopreserved using rapid and slow freezing procedures; however, variability in the success of replicates has limited its practical application. We have evaluated the potentially beneficial effects of adding 1 mg/ml of the polymer polyethylene glycol (PEG) (M(r) 8000) to a 6 M dimethyl sulfoxide (Me2SO) vitrification solution. Stepwise addition of cryoprotectant, either with or without PEG, was performed at room temperature (19-21 degrees C). Oocytes were then loaded in plastic insemination straws and held in liquid nitrogen vapour at -140 degrees C for 3 min prior to storage in liquid nitrogen. Oocytes were warmed rapidly to room temperature and removal of cryoprotective agent was effected in the presence of 1 M sucrose solution. Viability was assessed by vitro fertilization. Oocytes cryopreserved after exposure to 6 M Me2SO in the absence of PEG showed 60% normality, 80% fertilization, and 55% development to blastocyst, median of 11 replicate experiments (191 oocytes). Individual replicates yield highly variable survival which ranged from 0 to 100%. The addition of PEG significantly improved oocyte normality to 95% (range 76-100%; median of 9 replicate experiments, 301 oocytes). Rates of fertilization (91%; 60-100%) and development of blastocyst (73%; 67-92) were also improved. The addition of 1 mg/ml PEG to a 6 M Me2SO solution resulted in greatly improved viability of oocytes following cryopreservation and vastly reduced the variability seen with Me2SO solution alone." @default.
• W2028931781 created "2016-06-24" @default.
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• W2028931781 date "1997-05-01" @default.
• W2028931781 modified "2023-10-14" @default.
• W2028931781 title "Vitrification of Mature Mouse Oocytes: Improved Results Following Addition of Polyethylene Glycol to a Dimethyl Sulfoxide Solution" @default.
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• W2028931781 doi "https://doi.org/10.1006/cryo.1997.2007" @default.
• W2028931781 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9160998" @default.
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