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• W2028989857 abstract "Ergosterol is the major sterol constituent of most fungi. Since it is present in negligible amounts in higher plants, it can be used as a chemical marker for the presence of fungal contamination. A number of different ergosterol assays have been developed for the quantification of fungi in various samples. The paper presents the development of a new method for ergosterol detection based on the combination of non-discriminating flash pyrolysis with gas chromatography/mass spectrometry (Py-GC/MS). The design of the non-discriminating Py-GC/MS systems assures efficient transfer of high-molecular-weight pyrolysis products to the GC column for separation, followed by analyte detection by MS. The method was tested on different types of samples, including baker's yeast (Saccharomyces cerevisiae), moldy bread, indoor dust, and a leaf infected with powdery mildew. Ergosterol was detected in all these samples at levels ranging from approximately 4 mg/g for the baker's yeast to approximately 6 microg/g for household dust. The main benefits of non-discriminating pyrolysis over other techniques include elimination of the need for sample preparation, small sample size required and short analysis time." @default.
• W2028989857 created "2016-06-24" @default.
• W2028989857 creator A5024794266 @default.
• W2028989857 creator A5062196666 @default.
• W2028989857 date "2006-10-01" @default.
• W2028989857 modified "2023-10-18" @default.
• W2028989857 title "Determination of ergosterol as an indicator of fungal biomass in various samples using non-discriminating flash pyrolysis" @default.
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• W2028989857 doi "https://doi.org/10.1016/j.chroma.2006.07.045" @default.
• W2028989857 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16889786" @default.
• W2028989857 hasPublicationYear "2006" @default.
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