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• W2028993908 abstract "A new type of microcarrier was prepared for anchorage-dependent animal cell culture. Similar to the commercial product Cytodex 1, the newcomer is also spherical in micro-size range with ionic group DEAE, but the matrix is konjac glucomannan (KGM) from a natural plant. The optimal anion exchange capacity (AEC) was found to be 2.1 to 2.4 mmol/g dry microspheres. Five cell lines were cultured in static mode with the KGM microcarriers, including Vero, CHO-K1, MDCK, Wish and L929 cells. These cells adhered well and grew to high cell density comparable with or even better than those did on Cytodex 1. A further comparison was made with Vero cell culture in spinner mode. It was found that Vero cells achieved more than 2 × 106 cells/ml, and showed faster cell adhesion and higher specific growth rate on the KGM microcarriers than on Cytodex 1. In 5 L bioreactor perfusion culture, Vero cells grew above 7.0 × 106 cells/ml and the rabies virus titer was about 7.7 lgLD50/ml, compared to Cytodex 1 in the same culture condition. The results suggest that the new type of microcarrier could be a promising candidate for various cell cultures." @default.
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• W2028993908 date "2015-04-01" @default.
• W2028993908 modified "2023-10-13" @default.
• W2028993908 title "Novel konjac glucomannan microcarriers for anchorage-dependent animal cell culture" @default.
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• W2028993908 doi "https://doi.org/10.1016/j.bej.2014.12.012" @default.
• W2028993908 hasPublicationYear "2015" @default.
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