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• W2028998906 abstract "A technique using 35SO42− was developed to assess the physiological state, with respect to division rate and rate of protein synthesis, of marine phytoplankton. Radiosulfur is incorporated predominantly into protein and was ≈ 50% of total sulfur uptake for Thalassiosira weissflogii (Grunow) G. Fryxell and Hasle, comb. nov. (= T. fluviatilis Hustedt). Rates of protein synthesis calculated from the rate of 35S incorporation (S-inc) agreed with measured rates during active cell growth, but generally exceeded those measured during the stationary phase. Positive correlations were found between S-inc and changes in cell biomass in batch culture. S-inc per cell declined as T. weissflogii progressed from the exponential to the late stationary phase. Cultures of Amphidinium carteri Hulburt, Dunaliella tertiolecta Butcher and Pavlova (= Monochrysis) lutheri (Droop) Green behaved similarly. Changes in S-inc detected the onset of the stationary phase during growth with nitrate at 18 μM at least 24 h before it was observed by measuring 14C uptake. Molar ratios of S-inc in the dark to S-inc in the light, and 14C uptake rate to S-inc by T. weissflogii were low during exponential growth and elevated during the stationary phase. Relationships were found between each of these ratios and division rates. Incubation of natural populations from coastal waters of Nova Scotia gave results comparable to those obtained in laboratory batch cultures." @default.
• W2028998906 created "2016-06-24" @default.
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• W2028998906 date "1981-04-01" @default.
• W2028998906 modified "2023-09-23" @default.
• W2028998906 title "Determination of the physiological state of marine phytoplankton by use of Radiosulfate incorporation" @default.
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• W2028998906 doi "https://doi.org/10.1016/0022-0981(81)90130-1" @default.
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