FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029056395> ?p ?o ?g. }

• W2029056395 endingPage "1378" @default.
• W2029056395 startingPage "1364" @default.
• W2029056395 abstract "Abstract Bone tissue engineering approaches increasingly focus on the use of mesenchymal stem cells (MSC). In most animal transplantation models MSC are isolated and expanded before auto cell transplantation which might be critical for clinical application in the future. Hence this study compares the potential of directly auto‐transplanted versus in vitro expanded MSC with or without bone morphogenetic protein‐2 (BMP‐2) to induce bone formation in a large volume ceramic bone substitute in the sheep model. MSC were isolated from bone marrow aspirates and directly auto‐transplanted or expanded in vitro and characterized using fluorescence activated cell sorting (FACS) and RT‐PCR analysis before subcutaneous implantation in combination with BMP‐2 and β‐tricalcium phosphate/hydroxyapatite (β‐TCP/HA) granules. Constructs were explanted after 1 to 12 weeks followed by histological and RT‐PCR evaluation. Sheep MSC were CD29 + , CD44 + and CD166 + after selection by Ficoll gradient centrifugation, while directly auto‐transplanted MSC‐populations expressed CD29 and CD166 at lower levels. Both, directly auto‐transplanted and expanded MSC, were constantly proliferating and had a decreasing apoptosis over time in vivo . Directly auto‐transplanted MSC led to de novo bone formation in a heterotopic sheep model using a β‐TCP/HA matrix comparable to the application of 60 μg/ml BMP‐2 only or implantation of expanded MSC. Bone matrix proteins were up‐regulated in constructs following direct auto‐transplantation and in expanded MSC as well as in BMP‐2 constructs. Up‐regulation was detected using immunohistology methods and RT‐PCR. Dense vascularization was demonstrated by CD31 immunohistology staining in all three groups. Ectopic bone could be generated using directly auto‐transplanted or expanded MSC with β‐TCP/HA granules alone. Hence BMP‐2 stimulation might become dispensable in the future, thus providing an attractive, clinically feasible approach to bone tissue engineering." @default.
• W2029056395 created "2016-06-24" @default.
• W2029056395 creator A5005107946 @default.
• W2029056395 creator A5008569109 @default.
• W2029056395 creator A5013561416 @default.
• W2029056395 creator A5017210472 @default.
• W2029056395 creator A5057430042 @default.
• W2029056395 creator A5062111817 @default.
• W2029056395 creator A5065097566 @default.
• W2029056395 creator A5068621433 @default.
• W2029056395 creator A5069245069 @default.
• W2029056395 creator A5072285421 @default.
• W2029056395 date "2011-05-30" @default.
• W2029056395 modified "2023-10-16" @default.
• W2029056395 title "Directly auto-transplanted mesenchymal stem cells induce bone formation in a ceramic bone substitute in an ectopic sheep model" @default.
• W2029056395 cites W1551461802 @default.
• W2029056395 cites W1655444266 @default.
• W2029056395 cites W174271120 @default.
• W2029056395 cites W1964674805 @default.
• W2029056395 cites W1974753138 @default.
• W2029056395 cites W1978244403 @default.
• W2029056395 cites W1979185843 @default.
• W2029056395 cites W1982941157 @default.
• W2029056395 cites W1985460230 @default.
• W2029056395 cites W1989616003 @default.
• W2029056395 cites W1992647962 @default.
• W2029056395 cites W2001213911 @default.
• W2029056395 cites W2006501004 @default.
• W2029056395 cites W2009139825 @default.
• W2029056395 cites W2015230404 @default.
• W2029056395 cites W2031158924 @default.
• W2029056395 cites W2033951230 @default.
• W2029056395 cites W2034148679 @default.
• W2029056395 cites W2040179642 @default.
• W2029056395 cites W2070975738 @default.
• W2029056395 cites W2074215290 @default.
• W2029056395 cites W2074289481 @default.
• W2029056395 cites W2089567126 @default.
• W2029056395 cites W2105290236 @default.
• W2029056395 cites W2107423725 @default.
• W2029056395 cites W2114695859 @default.
• W2029056395 cites W2130023694 @default.
• W2029056395 cites W2143035259 @default.
• W2029056395 cites W2154790263 @default.
• W2029056395 cites W2169341720 @default.
• W2029056395 cites W2413969177 @default.
• W2029056395 cites W4244195039 @default.
• W2029056395 cites W4295850198 @default.
• W2029056395 cites W4376595816 @default.
• W2029056395 doi "https://doi.org/10.1111/j.1582-4934.2010.01131.x" @default.
• W2029056395 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4373324" @default.
• W2029056395 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20636333" @default.
• W2029056395 hasPublicationYear "2011" @default.
• W2029056395 type Work @default.
• W2029056395 sameAs 2029056395 @default.
• W2029056395 citedByCount "54" @default.
• W2029056395 countsByYear W20290563952012 @default.
• W2029056395 countsByYear W20290563952013 @default.
• W2029056395 countsByYear W20290563952014 @default.
• W2029056395 countsByYear W20290563952015 @default.
• W2029056395 countsByYear W20290563952016 @default.
• W2029056395 countsByYear W20290563952017 @default.
• W2029056395 countsByYear W20290563952018 @default.
• W2029056395 countsByYear W20290563952019 @default.
• W2029056395 countsByYear W20290563952020 @default.
• W2029056395 countsByYear W20290563952021 @default.
• W2029056395 countsByYear W20290563952023 @default.
• W2029056395 crossrefType "journal-article" @default.
• W2029056395 hasAuthorship W2029056395A5005107946 @default.
• W2029056395 hasAuthorship W2029056395A5008569109 @default.
• W2029056395 hasAuthorship W2029056395A5013561416 @default.
• W2029056395 hasAuthorship W2029056395A5017210472 @default.
• W2029056395 hasAuthorship W2029056395A5057430042 @default.
• W2029056395 hasAuthorship W2029056395A5062111817 @default.
• W2029056395 hasAuthorship W2029056395A5065097566 @default.
• W2029056395 hasAuthorship W2029056395A5068621433 @default.
• W2029056395 hasAuthorship W2029056395A5069245069 @default.
• W2029056395 hasAuthorship W2029056395A5072285421 @default.
• W2029056395 hasBestOaLocation W20290563952 @default.
• W2029056395 hasConcept C104317684 @default.
• W2029056395 hasConcept C126322002 @default.
• W2029056395 hasConcept C142724271 @default.
• W2029056395 hasConcept C16685009 @default.
• W2029056395 hasConcept C198826908 @default.
• W2029056395 hasConcept C202751555 @default.
• W2029056395 hasConcept C2780007613 @default.
• W2029056395 hasConcept C2780932548 @default.
• W2029056395 hasConcept C28328180 @default.
• W2029056395 hasConcept C2911091166 @default.
• W2029056395 hasConcept C30352789 @default.
• W2029056395 hasConcept C31507581 @default.
• W2029056395 hasConcept C55493867 @default.
• W2029056395 hasConcept C71924100 @default.
• W2029056395 hasConcept C86803240 @default.
• W2029056395 hasConcept C95444343 @default.
• W2029056395 hasConceptScore W2029056395C104317684 @default.
• W2029056395 hasConceptScore W2029056395C126322002 @default.
• W2029056395 hasConceptScore W2029056395C142724271 @default.

• next