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• W2029072842 startingPage "523" @default.
• W2029072842 abstract "We have investigated the response of four human cell lines, representing a range of sensitivities to ionizing radiation, to enzymes which induce defined DNA double-strand breaks (dsbs). Cell lines were derived from a normal individual, from the cancer-prone disorders ataxia-telangiectasia (AT) and Bloom's syndrome (BS), and from an immunodeficient individual (46BR). The molecular defects in AT and BS are unknown, while 46BR is known to be DNA ligase I deficient. We assayed the clonogenic survival of the cell lines following in vivo scission of the DNA by the restriction endonucleases PvuII and BanI. These two enzymes differ in their action; PvuII gives rise to dsbs with blunt termini, while BanI generates staggered ends with a 4 bp overhang. We found a correlation between the sensitivity of the cell lines to X-rays and to the blunt-end cutter PvuII, but not to the cohesive-end cutter BanI." @default.
• W2029072842 created "2016-06-24" @default.
• W2029072842 creator A5078271249 @default.
• W2029072842 creator A5091583144 @default.
• W2029072842 date "1993-01-01" @default.
• W2029072842 modified "2023-09-24" @default.
• W2029072842 title "Response of Radiation-sensitive Human Cells to Defined DNA Breaks" @default.
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• W2029072842 doi "https://doi.org/10.1080/09553009314551731" @default.
• W2029072842 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7902391" @default.
• W2029072842 hasPublicationYear "1993" @default.
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