FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029088142> ?p ?o ?g. }

• W2029088142 endingPage "639" @default.
• W2029088142 startingPage "625" @default.
• W2029088142 abstract "Protein storage vacuoles (PSVs) are specialized vacuoles devoted to the accumulation of large amounts of protein in the storage tissues of plants. In this study, we investigated the presence of the storage vacuole and protein trafficking to the compartment in cells of tobacco (Nicotiana tabacum), common bean (Phaseolus vulgaris), and Arabidopsis leaf tissue. When we expressed phaseolin, the major storage protein of common bean, or an epitope-tagged version of alpha-tonoplast intrinsic protein (alpha-TIP, a tonoplast aquaporin of PSV), in protoplasts derived from leaf tissues, these proteins were targeted to a compartment ranging in size from 2 to 5 microm in all three plant species. Most Arabidopsis leaf cells have one of these organelles. In contrast, from one to five these organelles occurred in bean and tobacco leaf cells. Also, endogenous alpha-TIP is localized in a similar compartment in untransformed leaf cells of common bean and is colocalized with transiently expressed epitope-tagged alpha-TIP. In Arabidopsis, phaseolin contained N-glycans modified by Golgi enzymes and its traffic was sensitive to brefeldin A. However, trafficking of alpha-TIP was insensitive to brefeldin A treatment and was not affected by the dominant-negative mutant of AtRab1. In addition, a modified alpha-TIP with an insertion of an N-glycosylation site has the endoplasmic reticulum-type glycans. Finally, the early step of phaseolin traffic, from the endoplasmic reticulum to the Golgi complex, required the activity of the small GTPase Sar1p, a key component of coat protein complex II-coated vesicles, independent of the presence of the vacuolar sorting signal in phaseolin. Based on these results, we propose that the proteins we analyzed are targeted to the PSV or equivalent organelle in leaf cells and that proteins can be transported to the PSV by two different pathways, the Golgi-dependent and Golgi-independent pathways, depending on the individual cargo proteins." @default.
• W2029088142 created "2016-06-24" @default.
• W2029088142 creator A5070035630 @default.
• W2029088142 creator A5079313764 @default.
• W2029088142 creator A5080425291 @default.
• W2029088142 creator A5083020137 @default.
• W2029088142 date "2004-02-01" @default.
• W2029088142 modified "2023-10-12" @default.
• W2029088142 title "Identification of the Protein Storage Vacuole and Protein Targeting to the Vacuole in Leaf Cells of Three Plant Species" @default.
• W2029088142 cites W1590397189 @default.
• W2029088142 cites W1844925061 @default.
• W2029088142 cites W1963980596 @default.
• W2029088142 cites W1965238703 @default.
• W2029088142 cites W1965556648 @default.
• W2029088142 cites W1965661381 @default.
• W2029088142 cites W1968568980 @default.
• W2029088142 cites W1972321507 @default.
• W2029088142 cites W1982557952 @default.
• W2029088142 cites W1983840712 @default.
• W2029088142 cites W1984315509 @default.
• W2029088142 cites W1987517959 @default.
• W2029088142 cites W2002250628 @default.
• W2029088142 cites W2005512591 @default.
• W2029088142 cites W2013883162 @default.
• W2029088142 cites W2021431752 @default.
• W2029088142 cites W2029329199 @default.
• W2029088142 cites W2053082803 @default.
• W2029088142 cites W2056921098 @default.
• W2029088142 cites W2058465353 @default.
• W2029088142 cites W2096053032 @default.
• W2029088142 cites W2098941202 @default.
• W2029088142 cites W2101693034 @default.
• W2029088142 cites W2104636880 @default.
• W2029088142 cites W2106996727 @default.
• W2029088142 cites W2108774790 @default.
• W2029088142 cites W2119879351 @default.
• W2029088142 cites W2120521842 @default.
• W2029088142 cites W2123775894 @default.
• W2029088142 cites W2126104240 @default.
• W2029088142 cites W2128897600 @default.
• W2029088142 cites W2129462035 @default.
• W2029088142 cites W2129583132 @default.
• W2029088142 cites W2132205053 @default.
• W2029088142 cites W2134225497 @default.
• W2029088142 cites W2136762525 @default.
• W2029088142 cites W2139646081 @default.
• W2029088142 cites W2141345634 @default.
• W2029088142 cites W2145778413 @default.
• W2029088142 cites W2147578072 @default.
• W2029088142 cites W2147588538 @default.
• W2029088142 cites W2150058998 @default.
• W2029088142 cites W2155616082 @default.
• W2029088142 cites W2156303060 @default.
• W2029088142 cites W2166027547 @default.
• W2029088142 cites W2170990177 @default.
• W2029088142 cites W2336000185 @default.
• W2029088142 cites W4230549533 @default.
• W2029088142 cites W4241129385 @default.
• W2029088142 cites W4243923626 @default.
• W2029088142 cites W4249895984 @default.
• W2029088142 doi "https://doi.org/10.1104/pp.103.030635" @default.
• W2029088142 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/344539" @default.
• W2029088142 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14730078" @default.
• W2029088142 hasPublicationYear "2004" @default.
• W2029088142 type Work @default.
• W2029088142 sameAs 2029088142 @default.
• W2029088142 citedByCount "120" @default.
• W2029088142 countsByYear W20290881422012 @default.
• W2029088142 countsByYear W20290881422013 @default.
• W2029088142 countsByYear W20290881422014 @default.
• W2029088142 countsByYear W20290881422015 @default.
• W2029088142 countsByYear W20290881422016 @default.
• W2029088142 countsByYear W20290881422017 @default.
• W2029088142 countsByYear W20290881422018 @default.
• W2029088142 countsByYear W20290881422019 @default.
• W2029088142 countsByYear W20290881422020 @default.
• W2029088142 countsByYear W20290881422021 @default.
• W2029088142 crossrefType "journal-article" @default.
• W2029088142 hasAuthorship W2029088142A5070035630 @default.
• W2029088142 hasAuthorship W2029088142A5079313764 @default.
• W2029088142 hasAuthorship W2029088142A5080425291 @default.
• W2029088142 hasAuthorship W2029088142A5083020137 @default.
• W2029088142 hasBestOaLocation W20290881422 @default.
• W2029088142 hasConcept C102568950 @default.
• W2029088142 hasConcept C104317684 @default.
• W2029088142 hasConcept C119062480 @default.
• W2029088142 hasConcept C143065580 @default.
• W2029088142 hasConcept C158617107 @default.
• W2029088142 hasConcept C168240541 @default.
• W2029088142 hasConcept C190062978 @default.
• W2029088142 hasConcept C2775988993 @default.
• W2029088142 hasConcept C2779491563 @default.
• W2029088142 hasConcept C2781373781 @default.
• W2029088142 hasConcept C55493867 @default.
• W2029088142 hasConcept C86803240 @default.
• W2029088142 hasConcept C95444343 @default.
• W2029088142 hasConcept C97564808 @default.
• W2029088142 hasConceptScore W2029088142C102568950 @default.

• next