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- W2029103693 abstract "CD8+ T lymphocytes are important mediators of adaptive immunity against certain viral, protozoan and bacterial pathogens. Activated CD8+ T cells are able to induce cytolysis of infected cells (perforin and CD95–CD95L mediated pathways) and also elaborate cytokines, including IFN-γ and TNF after appropriate MHC class I-peptide recognition. New technologies for the detection of antigen-specific CD8+ T cells, including tetrameric MHC class I-peptide complexes, intracellular IFN-γ staining and IFN-γ ELISPOT analysis have revised our understanding of the magnitude of the CD8+ T cell response to infection. Here, using intracellular cytokine staining, we compare detection of IFN-γ and TNF in the analysis of pathogen-specific CD8+ T cell lines and CD8+ T cells after primary viral infection (LCMV) or secondary bacterial infection (Listeria monocytogenes). Under multiple conditions and with multiple epitopes, we find that staining for intracellular IFN-γ consistently detects a higher frequency of antigen-specific CD8+ T cells than detection of intracellular TNF. However, (a) intracellular staining for TNF can be used to detect antigen-specific CD8+ T cell responses and (b) intracellular staining for cytokines is a useful approach for in vitro characterization of antigen-specific CD8+ T cell lines." @default.
- W2029103693 created "2016-06-24" @default.
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- W2029103693 date "2000-04-01" @default.
- W2029103693 modified "2023-10-18" @default.
- W2029103693 title "Intracellular staining for TNF and IFN-γ detects different frequencies of antigen-specific CD8+ T cells" @default.
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- W2029103693 doi "https://doi.org/10.1016/s0022-1759(00)00153-8" @default.
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