FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2029105004> ?p ?o ?g. }

• W2029105004 endingPage "1100" @default.
• W2029105004 startingPage "1085" @default.
• W2029105004 abstract "NRAMPs (natural resistance-associated macrophage proteins) have been characterized in mammals as divalent transition metal transporters involved in iron metabolism and host resistance to certain pathogens. The mechanism of pathogen resistance is proposed to involve sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. NRAMP homologues are also present in bacteria. We report the cloning and characterization of the single NRAMP genes in Escherichia coli and Salmonella enterica ssp. typhimurium, and the cloning of two distinct NRAMP genes from Pseudomonas aeruginosa and an internal fragment of an NRAMP gene in Burkholderia cepacia. The genes are designated mntH because the two enterobacterial NRAMPs encode H+-stimulated, highly selective manganese(II) transport systems, accounting for all Mn2+ uptake in each species under the conditions tested. For S. typhimurium MntH, the Km for 54Mn2+ ( approximately 0.1 microM) was pH independent, but maximal uptake increased as pH decreased. Monovalent cations, osmotic strength, Mg2+ and Ca2+ did not inhibit 54Mn2+ uptake. Ni2+, Cu2+ and Zn2+ inhibited uptake with Kis greater than 100 microM, Co2+ with a Ki of 20 microM and Fe2+ with a Ki that decreased from 100 microM at pH 7. 6 to 10 microM at pH 5.5. Fe3+ and Pb2+ inhibited weakly, exhibiting Kis of 50 microM, while Cd2+ was a potent inhibitor with a Ki of about 1 microM. E. coli MntH had a similar inhibition profile, except that Kis were three- to 10-fold higher. Both S. typhimurium and E. coli MntH also transport 55Fe2+ however, the Kms are equivalent to the Kis for Fe2+ inhibition of Mn2+ uptake, and are thus too high to be physiologically relevant. In both S. typhimurium and E. coli, mntH:lacZ constructs were strongly induced by hydrogen peroxide, weakly induced by EDTA and unresponsive to paraquat, consistent with the presence of Fur and OxyR binding sites in the promoters. Strains overexpressing mntH were more susceptible to growth inhibition by Mn2+ and Cd2+ than wild type, and strains lacking a functional mntH gene were more susceptible to killing by hydrogen peroxide. In S. typhimurium strain SL1344, mntH mutants showed no defect in invasion of or survival in cultured HeLa or RAW264.7 macrophage cells; however, expression of mntH:lacZ was induced severalfold by 3 h after invasion of the macrophages. S. typhimurium mntH mutants showed only a slight attenuation of virulence in BALB/c mice. Thus, the NRAMP Mn2+ transporter MntH and Mn2+ play a role in bacterial response to reactive oxygen species and possibly have a role in pathogenesis." @default.
• W2029105004 created "2016-06-24" @default.
• W2029105004 creator A5030919684 @default.
• W2029105004 creator A5036915664 @default.
• W2029105004 creator A5050707540 @default.
• W2029105004 creator A5062684664 @default.
• W2029105004 date "2000-06-01" @default.
• W2029105004 modified "2023-10-10" @default.
• W2029105004 title "The NRAMP proteins of Salmonella typhimurium and Escherichia coli are selective manganese transporters involved in the response to reactive oxygen" @default.
• W2029105004 cites W1236540755 @default.
• W2029105004 cites W1489896588 @default.
• W2029105004 cites W1533928907 @default.
• W2029105004 cites W1568628619 @default.
• W2029105004 cites W1582185972 @default.
• W2029105004 cites W1776345704 @default.
• W2029105004 cites W1787160128 @default.
• W2029105004 cites W1792710261 @default.
• W2029105004 cites W1803682227 @default.
• W2029105004 cites W1836520876 @default.
• W2029105004 cites W1872035403 @default.
• W2029105004 cites W1900832784 @default.
• W2029105004 cites W1910590028 @default.
• W2029105004 cites W1917523783 @default.
• W2029105004 cites W1938344531 @default.
• W2029105004 cites W1947164797 @default.
• W2029105004 cites W1958035891 @default.
• W2029105004 cites W1963789578 @default.
• W2029105004 cites W1967676653 @default.
• W2029105004 cites W1970793802 @default.
• W2029105004 cites W1970910747 @default.
• W2029105004 cites W1978836455 @default.
• W2029105004 cites W1983810303 @default.
• W2029105004 cites W1986509587 @default.
• W2029105004 cites W1994820064 @default.
• W2029105004 cites W2004186508 @default.
• W2029105004 cites W2004438884 @default.
• W2029105004 cites W2006089006 @default.
• W2029105004 cites W2007586351 @default.
• W2029105004 cites W2014958060 @default.
• W2029105004 cites W2021131973 @default.
• W2029105004 cites W2022836866 @default.
• W2029105004 cites W2032078625 @default.
• W2029105004 cites W2038142268 @default.
• W2029105004 cites W2039332574 @default.
• W2029105004 cites W2039634619 @default.
• W2029105004 cites W2043281920 @default.
• W2029105004 cites W2043841973 @default.
• W2029105004 cites W2048714638 @default.
• W2029105004 cites W2055276976 @default.
• W2029105004 cites W2059041636 @default.
• W2029105004 cites W2070132025 @default.
• W2029105004 cites W2070236309 @default.
• W2029105004 cites W2070330324 @default.
• W2029105004 cites W2072127906 @default.
• W2029105004 cites W2073475312 @default.
• W2029105004 cites W2074631079 @default.
• W2029105004 cites W2074744472 @default.
• W2029105004 cites W2077434907 @default.
• W2029105004 cites W2078208374 @default.
• W2029105004 cites W2084920157 @default.
• W2029105004 cites W2090401018 @default.
• W2029105004 cites W2090436685 @default.
• W2029105004 cites W2094122219 @default.
• W2029105004 cites W2096212458 @default.
• W2029105004 cites W2101806064 @default.
• W2029105004 cites W2103998251 @default.
• W2029105004 cites W2109452584 @default.
• W2029105004 cites W2111248422 @default.
• W2029105004 cites W2115235932 @default.
• W2029105004 cites W2120684043 @default.
• W2029105004 cites W2125578899 @default.
• W2029105004 cites W2129676027 @default.
• W2029105004 cites W2131215925 @default.
• W2029105004 cites W2136107297 @default.
• W2029105004 cites W2136451832 @default.
• W2029105004 cites W2137143414 @default.
• W2029105004 cites W2137314317 @default.
• W2029105004 cites W2140901337 @default.
• W2029105004 cites W2141902291 @default.
• W2029105004 cites W2142009418 @default.
• W2029105004 cites W2146903969 @default.
• W2029105004 cites W2149626409 @default.
• W2029105004 cites W2150019542 @default.
• W2029105004 cites W2157103734 @default.
• W2029105004 cites W2159789689 @default.
• W2029105004 cites W2228239024 @default.
• W2029105004 cites W2465826357 @default.
• W2029105004 cites W261351262 @default.
• W2029105004 cites W4245747415 @default.
• W2029105004 cites W4301101990 @default.
• W2029105004 cites W60527679 @default.
• W2029105004 cites W88493969 @default.
• W2029105004 doi "https://doi.org/10.1046/j.1365-2958.2000.01922.x" @default.
• W2029105004 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10844693" @default.
• W2029105004 hasPublicationYear "2000" @default.
• W2029105004 type Work @default.
• W2029105004 sameAs 2029105004 @default.
• W2029105004 citedByCount "274" @default.

• next