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• W2029120593 abstract "An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved that makes it possible to selectively and efficiently incorporate para-cyanophenylalanine (pCNPhe) into proteins in E. coli at sites specified by the amber nonsense codon, TAG. Substitution of pCNPhe for histidine-64 in myoglobin (Mb) affords a sensitive vibrational probe of ligand binding. This methodology provides a useful infrared reporter of protein structure, biomolecular interactions, and conformational changes." @default.
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• W2029120593 date "2006-10-12" @default.
• W2029120593 modified "2023-10-13" @default.
• W2029120593 title "A Genetically Encoded Infrared Probe" @default.
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• W2029120593 doi "https://doi.org/10.1021/ja0636690" @default.
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