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- W2029122647 abstract "Peptidoglutaminase-I and II that catalyzed the hydrolysis of the γ-amide of peptidebound glutamine, were purified from the cell-free extracts of Bacillus circulans by streptomycin sulfate precipitation, ammonium sulfate fractionation, DEAE-Sephadex, Sephadex G-200, QAE-Sephadex, hydroxylapatite-cellulose column chromatography, and finally preparative polyacrylantide gel disc electrophoresis. The purification steps resultd in a 714-fold increase in specific activity for peptidoglutaminase-I and in a 223-fold for peptidoglutaminase-II over the original extracts. The both enzymes were homogeneous in disc electrophoresis in polyacrylamide gel, immunoelectrophoresis in agar gel, and sedimentation analysis. Using gel filtration, the molecular weights of peptidoglutaminases I and II were estimated to be 90,000 and 125,000. However, during the purification steps, the both enzymes were observed to cause the dissociation and aggregation reaction which did not so much affect on their enzyme activities." @default.
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- W2029122647 date "1973-01-01" @default.
- W2029122647 modified "2023-10-16" @default.
- W2029122647 title "Peptidoglutaminase-I and II: Isolation in Homogeneous Form" @default.
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- W2029122647 doi "https://doi.org/10.1271/bbb1961.37.827" @default.
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