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• W2029126601 abstract "The bacterial killing efficiency of a dynamic nanoplatform (DNP) was evaluated. The polyacrylamide (PAA) hydrogel matrix of the DNP was loaded with methylene blue (MB) and was previously applied successfully to killing rat C6 glioma tumor cells in culture. This series of experiments is aimed at determining the suitability of this nanoplatform for elimination of bacterial infections. Suspended cultures of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Acinetobacter sp. were exposed to activated ( approximately 650-nm laser light) MB-PAA-DNPs. The killing efficiency of nanoparticle mass concentration, light irradiance and fluence, and dark incubation time was determined on each of the bacterial species. Moreover, the ability of activated MB-PAA-DNPs to inhibit biofilm growth and eradicate and disperse preformed biofilms, preformed on glass and polystyrene surfaces, was demonstrated. The data revealed that activated MB-PAA-DNPs eradicated all species of bacteria examined. Also, encapsulation of MB into the PAA-DNP matrix significantly diminished the observed dark toxicity of free dye. The photobactericidal efficacy of MB-PAA-DNP was found to be higher for gram-positive bacteria than for gram-negative bacteria. In addition, activated MB-PAA-DNP can inhibit biofilm growth and eradicate almost all of the early-age biofilms that are formed by all of the bacteria examined." @default.
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• W2029126601 date "2009-07-01" @default.
• W2029126601 modified "2023-10-16" @default.
• W2029126601 title "Eradication of Bacteria in Suspension and Biofilms Using Methylene Blue-Loaded Dynamic Nanoplatforms" @default.
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• W2029126601 doi "https://doi.org/10.1128/aac.01604-08" @default.
• W2029126601 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2704698" @default.
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