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- W2029127545 abstract "In this paper the development and feasibility of a novel detection system for a low molecular weight chemical, in which a peptide was utilized as a binding molecule, are described. Surface plasmon resonance (SPR) apparatus was used as a transducer. The porphyrin binding peptide, PSP2, was used as a model peptide ligand, while a porphyrin derivative, H2TMpyP, was used as a model low-molecular-weight chemical. PSP2 was covalently immobilized onto the SPR sensor chip and SPR measurement using the PSP2-immobilized chip for various concentrations of porphyrin was carried out. H2TMpyP was detectable in the range from 100 ng ml−1 to 10 μg ml−1 with a linear correlation and good precision and the PSP2-immobilized chip could be regenerated within 1 min after measurement in this system. From comparison of the detection manners of three porphyrin derivatives, the ability of a short peptide to discriminate between differences in molecular structure was demonstrated. Moreover, the self-assembled monolayer (SAM) of PSP2 was successfully prepared on the gold substrate and H2TMpyP could be detected using the PSP2-SAM chip." @default.
- W2029127545 created "2016-06-24" @default.
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- W2029127545 date "2001-12-01" @default.
- W2029127545 modified "2023-10-14" @default.
- W2029127545 title "Detection of porphyrin using a short peptide immobilized on a surface plasmon resonance sensor chip" @default.
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- W2029127545 doi "https://doi.org/10.1016/s0956-5663(01)00188-9" @default.
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