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- W2029132406 abstract "Rat rod outer segments (ROSs) were isolated by vortexing retinas and separating the detached components on preformed Percoll gradients. A lighter band of 20 × 106 unsealed ROSs per ten retinas, and a heavier band of 60 × 106 sealed ROSs per ten retinas were obtained from each 12 ml gradient. The yield of sealed ROSs (but not unsealed ROSs) was increased up to twofold in the presence of the specific cysteine protease inhibitor, Ep-475. Aprotinin, pepstatin, PMSF, TPCK and EGTA plus EDTA had no effect. These results indicate that during isolation, ROSs are vulnerable to damage by cysteine protease activity either from damaged retinal cells or from within." @default.
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- W2029132406 date "1989-09-01" @default.
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- W2029132406 title "Isolation of rod outer segments on Percoll gradients: Effect of specific protease inhibition" @default.
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- W2029132406 doi "https://doi.org/10.1016/0014-4835(89)90052-3" @default.
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