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- W2029134463 abstract "ABSTRACT The ability to express and purify large quantity of proteins in bacteria has greatly impacted many aspects of biological research. These include their use as a source of reagent for biochemical and biophysical studies as well as a source of antigen for antibody production. Currently many different expression systems are available and new ones are being developed. These systems allow inducible expression of a desired protein as a fusion with an affinity tag for simple purification. The affinity tags can generally be removed by specific proteases which recognize cleavage sites engineered between the affinity tag and the desired protein. Presence of tags that encode epitopes of specific antibodies provide additional means for identification of recombinant proteins. This review provides an overview of some of the most commonly utilized expression systems and examples of the use of these proteins in biochemical and biophysical studies. I will also describe other available systems which may provide suitable alternative for expression of recombinant proteins." @default.
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- W2029134463 date "1999-01-01" @default.
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- W2029134463 title "Prokaryotic Gene Fusion Expression Systems and their Use in Structural and Functional Studies of Proteins" @default.
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- W2029134463 doi "https://doi.org/10.1080/10826069908544695" @default.
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