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• W2029145691 abstract "Deoxyribonucleic acid extracted from a clear plaque mutant of the temperate phage, SPO2, was infectious when incubated with competent cultures of Bacillus subtilis 168 M. The relationship between numbers of infectious centers and DNA concentration was linear. The sensitivity of the infectious DNA to ultraviolet light was much greater than that of the free phage when B. subtilis 168 M was used as host. Acriflavin, which inhibits host cell reactivation, increased the rate of ultraviolet inactivation of the free phage so that it approached the inactivation rate of the phage DNA. Acriflavin had little effect on the survival curves of the infectious DNA. Non-host reactivating mutants (hcr−)† of B. subtilis 168 M were isolated. The survival curves of SPO2 phage were much steeper when the hcr− mutant was used as a host than those obtained when the hcr+ parental strain was used as a host. Ultraviolet sensitivity of the phage DNA was still greater than that of the free phage even when B. subtilis hcr− was used as host, but the difference in sensitivity was much less than the difference obtained with B. subtilis hcr+ as a host. Possible explanations for the greater ultraviolet sensitivity of the infectious DNA are discussed." @default.
• W2029145691 created "2016-06-24" @default.
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• W2029145691 date "1965-11-01" @default.
• W2029145691 modified "2023-09-23" @default.
• W2029145691 title "Comparison of ultraviolet sensitivity of Bacillus subtilis bacteriophage SPO2 and its infectious DNA" @default.
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• W2029145691 doi "https://doi.org/10.1016/s0022-2836(65)80235-2" @default.
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